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Titolo:
Chromosomal influence on meiotic spindle assembly: Abnormal meiosis I in female Mlh1 mutant mice
Autore:
Woods, LM; Hodges, CA; Baart, E; Baker, SM; Liskay, M; Hunt, PA;
Indirizzi:
Case Western Reserve Univ, Dept Genet, Sch Med, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Case Western Reserve Univ, Ctr Human Genet, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Univ Hosp Cleveland, Cleveland, OH 44106 USA Univ Hosp Cleveland Cleveland OH USA 44106 eland, Cleveland, OH 44106 USA Agr Univ Wageningen, Dept Anim Breeding, NL-6709 PG Wageningen, Netherlands Agr Univ Wageningen Wageningen Netherlands NL-6709 PG ingen, Netherlands Univ Calif Berkeley, Div Nutrit Sci & Toxicol, Berkeley, CA 94720 USA UnivCalif Berkeley Berkeley CA USA 94720 Toxicol, Berkeley, CA 94720 USA Oregon Hlth Sci Univ, Dept Mol & Med Genet, Portland, OR 97201 USA Oregon Hlth Sci Univ Portland OR USA 97201 Genet, Portland, OR 97201 USA
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 7, volume: 145, anno: 1999,
pagine: 1395 - 1406
SICI:
0021-9525(19990628)145:7<1395:CIOMSA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CYCLE CHECKPOINTS; DNA MISMATCH REPAIR; MOUSE OOCYTES; TRISOMY FORMATION; CROSSING-OVER; KINETOCHORE; MATURATION; MECHANISM; RECOMBINATION; METAPHASE;
Keywords:
cell cycle; meiosis; mismatch repair; oocyte; spindle formation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Hunt, PA Case Western Reserve Univ, Dept Genet, Sch Med, 10900 Euclid Ave,Cleveland, OH 44106 USA Case Western Reserve Univ 10900 Euclid Ave Cleveland OH USA 44106
Citazione:
L.M. Woods et al., "Chromosomal influence on meiotic spindle assembly: Abnormal meiosis I in female Mlh1 mutant mice", J CELL BIOL, 145(7), 1999, pp. 1395-1406

Abstract

In mouse oocytes, the first meiotic spindle is formed through the action of multiple microtubule organizing centers rather than a pair of centrosomes. Although the chromosomes are thought to play a major role in organizing the meiotic spindle, it remains unclear how a stable bipolar spindle is established. We have studied the formation of the first meiotic spindle in murine oocytes from mice homozygous for a targeted disruption of the DNA mismatch repair gene, Mlh1. In the absence of the MLH1 protein meiotic recombination is dramatically reduced and, as a result, the vast majority of chromosomes are present as unpaired univalents at the first meiotic division. The orientation of these univalent chromosomes at prometaphase suggests that they are unable to establish stable bipolar spindle attachments, presumably due to the inability to differentiate functional kinetochore domains on individual sister chromatids. In the presence of this aberrant chromosome behavior a stable first meiotic spindle is not formed, the spindle poles continueto elongate, and the vast majority of cells never initiate anaphase. Theseresults suggest that, in female meiotic systems in which spindle formationis based on the action of multiple microtubule organizing centers, the chromosomes not only promote microtubule polymerization and organization but their attachment to opposite spindle poles acts to stabilize the forming spindle poles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 20:21:49