Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Preparation and functional properties of monoclonal antibodies to human, mouse and rat OX-2
Autore:
Ragheb, R; Abrahams, S; Beecroft, R; Hu, J; Ni, J; Ramakrishna, V; Yu, G; Gorczynski, RM;
Indirizzi:
Toronto Hosp, Transplant Res Div, Toronto, ON M5G 2C4, Canada Toronto Hosp Toronto ON Canada M5G 2C4 s Div, Toronto, ON M5G 2C4, Canada Bio Can Sci Inc, Mississauga, ON, Canada Bio Can Sci Inc Mississauga ON Canada n Sci Inc, Mississauga, ON, Canada Immuno Precise Antibodies Ltd, Victoria, BC, Canada Immuno Precise Antibodies Ltd Victoria BC Canada d, Victoria, BC, Canada Univ Toronto, Dept Surg, Toronto, ON, Canada Univ Toronto Toronto ON Canada v Toronto, Dept Surg, Toronto, ON, Canada Univ Toronto, Dept Immunol, Toronto, ON, Canada Univ Toronto Toronto ON Canada oronto, Dept Immunol, Toronto, ON, Canada
Titolo Testata:
IMMUNOLOGY LETTERS
fascicolo: 2-3, volume: 68, anno: 1999,
pagine: 311 - 315
SICI:
0165-2478(19990601)68:2-3<311:PAFPOM>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORTAL VENOUS IMMUNIZATION; GRAFT PROLONGATION; INTERLEUKIN-12; COMBINATION; CELLS;
Keywords:
cytokine production; human; hybridoma; monoclonal antibodies; mouse; OX-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Gorczynski, RM Toronto Hosp, Transplant Res Div, 200 Elizabeth St,CCRW 2-855, Toronto, ONM5G 2C4, Canada Toronto Hosp 200 Elizabeth St,CCRW 2-855 Toronto ON Canada M5G 2C4
Citazione:
R. Ragheb et al., "Preparation and functional properties of monoclonal antibodies to human, mouse and rat OX-2", IMMUNOL LET, 68(2-3), 1999, pp. 311-315

Abstract

We have prepared mouse and rat hybridomas to a 43-kDa molecule expressed in the thymus, on a subpopulation of dendritic cells, and in the brain, in mammalian tissue derived from mouse, rat and human. Using CHO cells transiently transfected with adenovirus vector(s) expressing a cDNA construct for the relevant OX-2 gene, we show these monoclonal antibodies (Mabs) detect a molecule encoded by this construct (rat OX-2 (rOX-2), mouse OX-2 (mOX-2) and human OX-2 (huOX-2), respectively). Furthermore, at least some of the anti-rat Mabs detect determinants expressed on the murine OX-2 molecule, as wepredicted in an earlier publication. Previous studies have implied that this molecule might serve an important role in regulation of cell signaling for cytokine production. Using one-way mixed leukocyte reactions we show that when cells are cultured in the presence of the species-specific Mab, cytokine production becomes polarized 'away from' type-2 cytokine production, with preferentially increased expression of type-1 cytokine production. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 08:56:33