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Titolo:
Correspondence of gradual developmental increases of expression of galectin-reactive glycoconjugates with alterations of the total contents of the two differentially regulated galectins in chicken intestine and liver as indication for overlapping functions
Autore:
Lips, KS; Kaltner, H; Reuter, G; Stierstorfer, B; Sinowatz, F; Gabius, HJ;
Indirizzi:
Univ Munich, Inst Physiol Chem, Tier Fak, D-80539 Munich, Germany Univ Munich Munich Germany D-80539 em, Tier Fak, D-80539 Munich, Germany Univ Munich, Inst Tieranat, Tier Fak, D-80539 Munich, Germany Univ MunichMunich Germany D-80539 at, Tier Fak, D-80539 Munich, Germany
Titolo Testata:
HISTOLOGY AND HISTOPATHOLOGY
fascicolo: 3, volume: 14, anno: 1999,
pagine: 743 - 760
SICI:
0213-3911(199907)14:3<743:COGDIO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
GALACTOSIDE-BINDING LECTINS; HUMAN APPENDIX VERMIFORMIS; ANIMAL LECTINS; ENDOGENOUS LECTIN; MISTLETOE LECTIN; AFFINITY-CHROMATOGRAPHY; CONFORMER SELECTION; RANDOM-WALK; MUSCLE; CELL;
Keywords:
agglutinin; embryogenesis; galectin; glycoprotein; intestine; lectin; liver;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
76
Recensione:
Indirizzi per estratti:
Indirizzo: Gabius, HJ Univ Munich, Inst Physiol Chem, Tier Fak, Vet Str 13, D-80539 Munich, Germany Univ Munich Vet Str 13 Munich Germany D-80539 Munich, Germany
Citazione:
K.S. Lips et al., "Correspondence of gradual developmental increases of expression of galectin-reactive glycoconjugates with alterations of the total contents of the two differentially regulated galectins in chicken intestine and liver as indication for overlapping functions", HIST HISTOP, 14(3), 1999, pp. 743-760

Abstract

The duplication of genes for recognition molecules and the ensuing diversification of the members of such families generate complex groups of homologous proteins. One example are galactoside-specific lectins whose sequences display constant features related to sugar binding, the galectins. Based onthe inverse abundance of the chicken galectins CG-14 and CG-16 in adult intestine and liver, these two lectins represent a model to comparatively study expression of the related proteins and the galectin-reactive sites (glycoproteins and glycolipids) biochemically and histochemically. Functional overlap and/or acquisition of distinct functions would be reflected in qualitative and/or quantitative aspects of ligand display. Using five different stages of embryogenesis, differential regulation of the two galectins was detected in liver and intestine. The clear preference for one galectin (CG-14) was observed in intestine already at rather early stages, whereas equivalence for both proteins was noted in liver from day 12 to day 18 prior to hatching, as seen by ELISA assays and Western blot analysis. Presentation of galectin-reactive glycoproteins showed a tendency for gradual increase in both organs. Galectin-blotting analysis revealed primarily very similar patterns of positive bands at the different stages of development and only few quantitative and qualitative changes. The reactivity of glycolipids in a solid-phase assay was more variable, even surpassing the response of extractsof the adult organ at several embryonic stages. While the localization patterns of the galectins and galectin-reactive sites were nearly indistinguishable in the liver, intestinal tissue differed with respect to the placement and accessibility of binding sites. Thus, the results suggest a differential regulation of galectin activities in the two organs. As a sum they resemble the course of development of availability of glycoprotein ligands in vitro. These findings support the notion for a partial functional redundancyin this family. The described approach to employ galectin-specific antibodies and the labeled galectins as tools to assess presentation of ligands issuggested to be of general relevance to address the question of distinct vs. overlapping functions of related recognition molecules.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:59:13