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Titolo:
Analysis of the in vivo interaction between a basic repressor and an acidic activator
Autore:
Wellhausen, A; Lehming, N;
Indirizzi:
Max Planck Gesell, Max Delbruck Lab, D-50829 Cologne, Germany Max Planck Gesell Cologne Germany D-50829 Lab, D-50829 Cologne, Germany
Titolo Testata:
FEBS LETTERS
fascicolo: 3, volume: 453, anno: 1999,
pagine: 299 - 304
SICI:
0014-5793(19990625)453:3<299:AOTIVI>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-POLYMERASE-II; YEAST TRANSCRIPTIONAL ACTIVATORS; IN-VIVO; SACCHAROMYCES-CEREVISIAE; HOLOENZYME; DROSOPHILA; COMPLEX; PROTEIN; GENES; COACTIVATOR;
Keywords:
repression; SSB24; GCN4; split ubiquitin assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Lehming, N Max Planck Gesell, Max Delbruck Lab, Carl Von Linne Weg 10, D-50829 Cologne, Germany Max Planck Gesell Carl Von Linne Weg 10 Cologne Germany D-50829
Citazione:
A. Wellhausen e N. Lehming, "Analysis of the in vivo interaction between a basic repressor and an acidic activator", FEBS LETTER, 453(3), 1999, pp. 299-304

Abstract

The artificial basic repressor SSB24 represses transcription of a reporterconstruct activated by GCN4. We show that the positively charged SSB24 andthe negatively charged acidic activator GCN4 interact in vitro and in vivo. However, deleting the interaction domain from the GCN4 activator does notresult in loss of repression by SSB24. Similarly, transcription activated by the holoenzyme component SRB2 is repressed, although SSB24 and SRB2 do not interact. Repression by SSB24 therefore does not depend on the observed protein-protein interaction between SSB24 and GCN4, (C) 1999 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 08:49:00