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Titolo:
Homologous analysis on centromeric region sequences of A and B chromosomesfrom rye by FISH
Autore:
Zhang, RX; Chen, CB; Song, WQ; Li, XL; Chen, RY;
Indirizzi:
Nankai Univ, Dept Biol, Tianjin 300071, Peoples R China Nankai Univ Tianjin Peoples R China 300071 anjin 300071, Peoples R China
Titolo Testata:
CHINESE SCIENCE BULLETIN
fascicolo: 8, volume: 44, anno: 1999,
pagine: 704 - 707
SICI:
1001-6538(199904)44:8<704:HAOCRS>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Keywords:
B chromosome of rye; centromeric region; microdissection; homology; FISH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, RY Nankai Univ, Dept Biol, Tianjin 300071, Peoples R China Nankai Univ Tianjin Peoples R China 300071 071, Peoples R China
Citazione:
R.X. Zhang et al., "Homologous analysis on centromeric region sequences of A and B chromosomesfrom rye by FISH", CHIN SCI B, 44(8), 1999, pp. 704-707

Abstract

Four centromeric segments from B chromosomes (Bs) of rye have been microdissected and amplified by linker adapter PCR (LA-PCR). The PCR products ranged from 100 to 2 000 bp, Fluorescence in situ hybridization (FISH) experiment has been carried out using PCR products as the probe, which was labeled by DIG-11-dUTP. The result confirms that these PCR products from Bs centromeric region are homologous with that of A chromosomes (As) in rye. It also proves that Bs are originated from As.

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Documento generato il 02/12/20 alle ore 17:34:27