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Titolo:
Characterization of the bacterial community of a zinc-polluted soil
Autore:
Brim, H; Heuer, H; Krogerrecklenfort, E; Mergeay, M; Smalla, K;
Indirizzi:
Vlaasme Instelling Technol Onderzoek, B-2400 Mol, Belgium Vlaasme Instelling Technol Onderzoek Mol Belgium B-2400 400 Mol, Belgium Biol Bundesanstalt Land & Forstwirtschaft, Inst Biochem & Pflanzenvirol, D-38104 Braunschweig, Germany Biol Bundesanstalt Land & Forstwirtschaft Braunschweig Germany D-38104 y ULB, Lab Genet Procaryotes, Dept Biol Mol, B-1640 Rhode St Genese, BelgiumULB Rhode St Genese Belgium B-1640 Mol, B-1640 Rhode St Genese, Belgium
Titolo Testata:
CANADIAN JOURNAL OF MICROBIOLOGY
fascicolo: 4, volume: 45, anno: 1999,
pagine: 326 - 338
SICI:
0008-4166(199904)45:4<326:COTBCO>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; GRADIENT GEL-ELECTROPHORESIS; 16S RIBOSOMAL-RNA; ALCALIGENES-EUTROPHUS CH34; HEAVY-METALS; MICROBIAL-POPULATIONS; RESISTANT-BACTERIA; DIRECT EXTRACTION; DNA EXTRACTION; NUCLEIC-ACIDS;
Keywords:
microbial community analysis; cultivation; 16S rDNA analysis; TGGE; sequencing; Zn-polluted soil;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Brim, H Uniformed Serv Univ Hlth Sci, Dept Pathol, 4301 Jones Bridge Rd, Bethesda,MD 20812 USA Uniformed Serv Univ Hlth Sci 4301 Jones Bridge Rd Bethesda MD USA 20812
Citazione:
H. Brim et al., "Characterization of the bacterial community of a zinc-polluted soil", CAN J MICRO, 45(4), 1999, pp. 326-338

Abstract

The bacterial community of a zinc-contaminated soil (Maatheide soil in Lommel, Belgium) was studied using cultivation as well as cultivation-independent techniques. Colony-forming units (CFU) were determined by plating on media with or without metals. Dominant isolates were characterized by fatty acid methyl ester analysis (FAME analysis) and PCR fingerprinting using repetitive extragenic palindromic sequences as primers. DNA was directly extracted from soil samples and used as a template for the PCR amplification of the 16S rDNA (8-1511) or a 16S rDNA fragment (968-1401). Clones resulting from cloning the 16S rDNA from soil DNA were sequenced. Temperature gradient gel electrophoresis (TGGE analysis) was performed for 16S rDNA fragments (968-1401) amplified from the dominant isolates, the clones, and the total soil DNA extracted according to two protocols differing in strength of lysis. Total CFU ranged from 10(4) to 10(5)/g soil. The majority of the isolates were identified by FAME analysis as Arthrobacter spp. (18 out of 23). None of the isolates were identified as a Ralstonia eutropha like strain (formerly Alcaligenes eutrophus). Metalloresistant Rastomia eutropha like strains were previously shown to be dominant in the analyzed biotope. Most of the isolates were zinc tolerant but only seven could be considered zinc resistant. Sequences of the 16S rDNA clones obtained from total soil DNA were affiliated with genes of different bacteria such as alpha-proteobacteria, beta-proteobacteria, and the Cytophaga-Flexibacter-Bacteroides group. None of thesequenced clones aligned with the Ralstonia eutropha 16S rRNA gene. TGGE analysis of the 16S rDNA fragments (968-1401) amplified from the dominant strains, the clones, and the total soil DNA showed that isolates and clones represented only a part of the bands present in the TGGE pattern from total DNA. The 968-1401 fragment amplified from all Arthrobacter strains had a similar electrophoretic mobility. This band was seen as a major band in the pattern of DNA extracted from soil using a harsh cell lysis, whereas it did not appear, or appeared only as a weak band, in patterns obtained from soilDNA extracted using gentle lysis. The previously reported predominance of a Ralstonia eutropha like strain in this soil was no longer observed. This may suggest a population replacement by less resistant bacteria, concomitant with a progressive decrease of the zinc toxicity in the Maatheide soil.

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Documento generato il 21/09/20 alle ore 18:43:28