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Titolo:
The increment of micronucleus frequency in cervical carcinoma during irradiation in vivo and its prognostic value for tumour radiocurability
Autore:
Widel, M; Jedrus, S; Owczarek, S; Konopacka, M; Lubecka, B; Kolosza, Z;
Indirizzi:
Marie Sklodowska Curie Inst Gliwice, Dept Expt & Clin Radiobiol, PL-44101 Gliwice, Poland Marie Sklodowska Curie Inst Gliwice Gliwice Poland PL-44101 wice, Poland Marie Sklodowska Curie Inst Gliwice, Clin Oncol Gynaecol, PL-44101 Gliwice, Poland Marie Sklodowska Curie Inst Gliwice Gliwice Poland PL-44101 wice, Poland Marie Sklodowska Curie Inst Gliwice, Ctr Oncol, Dept Epidemiol, PL-44101 Gliwice, Poland Marie Sklodowska Curie Inst Gliwice Gliwice Poland PL-44101 wice, Poland
Titolo Testata:
BRITISH JOURNAL OF CANCER
fascicolo: 10, volume: 80, anno: 1999,
pagine: 1599 - 1607
SICI:
0007-0920(199907)80:10<1599:TIOMFI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-LINES; INTRINSIC RADIOSENSITIVITY; RADIATION SENSITIVITY; PATIENT RESPONSE; RADIOTHERAPY; SURVIVAL; ASSAY; PREDICTION; FRACTION;
Keywords:
cervical carcinoma; radiotherapy; micronucleus assay; predictive value;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Widel, M Marie Sklodowska Curie Inst Gliwice, Dept Expt & Clin Radiobiol, 15 Armii Krajowej Str, PL-44101 Gliwice, Poland Marie Sklodowska Curie InstGliwice 15 Armii Krajowej Str Gliwice Poland PL-44101
Citazione:
M. Widel et al., "The increment of micronucleus frequency in cervical carcinoma during irradiation in vivo and its prognostic value for tumour radiocurability", BR J CANC, 80(10), 1999, pp. 1599-1607

Abstract

A potential usefulness of micronucleus assay for prediction of tumour radiosensitivity has been tested in 64 patients with advanced stage (II B-IV B)cervical carcinoma treated by radiotherapy. The study of cellular radiosensitivity in vitro was conducted in parallel with the study of cellular damage after tumour irradiation in vivo. Radiosensitivity of in vitro cultured primary cells isolated from tumour biopsies taken before radiotherapy was evaluated using cytokinesis-block micronucleus assay. Frequency of micronuclei per binucleated cell (MN/BNC) at 2 Gy was used as a measure of radiosensitivity. Radiation sensitivity in vivo was expressed as per cent increment of micronucleus frequency in cells isolated from biopsy taken after 20 Gy (external irradiation, 10 x 2 Gy) over the pre-treatment spontaneous micronucleus level and was called MN20. Very low correlation (r = 0.324) was observed between micronucleus frequency in vitro and in vivo. Although micronucleus frequency at 2 Gy differed widely between tumours evaluated (mean MN/BNC was 0.224; range 0.08-0.416), no significant correlation was observed between this parameter and clinical outcome. The average increment of micronucleus frequency after 20 Gy amounted to 193% of spontaneous level (range 60-610%) and was independent of spontaneous micronucleation before radiotherapy. In contrast to in vitro results, these from in vivo assay seem to have apredictive value for radiotherapy of cervix cancer. The micronucleus increment in vivo that reached at least 117.5% of pretreatment value (first quartile for MN20 data set) correlated significantly with better tumour local control (P < 0.008) and overall survival (P< 0.045). Our results suggest that evaluation of increment of micronucleus frequency during radiotherapy (after fixed tested dose of 20 Gy) offers a potentially valuable approach to predicting individual radioresponsiveness and may be helpful for individualization of treatment strategy in advanced stage cervical cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:30:53