Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Interplay of maturation-promoting factor and mitogen-activated protein kinase inactivation during metaphase-to-interphase transition of activated bovine oocytes
Autore:
Liu, L; Yang, XZ;
Indirizzi:
Univ Connecticut, Dept Anim Sci, Storrs, CT 06269 USA Univ Connecticut Storrs CT USA 06269 Dept Anim Sci, Storrs, CT 06269 USA
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 1, volume: 61, anno: 1999,
pagine: 1 - 7
SICI:
0006-3363(199907)61:1<1:IOMFAM>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
DUAL-SPECIFICITY PHOSPHATASE; MATURED IN-VITRO; MAP KINASE; XENOPUS-OOCYTE; MOUSE OOCYTES; PARTHENOGENETIC DEVELOPMENT; MEIOTIC MATURATION; 6-DIMETHYLAMINOPURINE 6-DMAP; TYROSINE PHOSPHORYLATION; POTENTIAL ROLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Yang, XZ Univ Connecticut, Dept Anim Sci, 3636 Horsebarn Rd,Ext 40, Storrs, CT 06269 USA Univ Connecticut 3636 Horsebarn Rd,Ext 40 Storrs CT USA 06269 USA
Citazione:
L. Liu e X.Z. Yang, "Interplay of maturation-promoting factor and mitogen-activated protein kinase inactivation during metaphase-to-interphase transition of activated bovine oocytes", BIOL REPROD, 61(1), 1999, pp. 1-7

Abstract

The objective of the present study was to examine the activity changes in histone H1 kinase (also known as maturation-promoting factor [MPF]) and mitogen-activated protein kinase (MAPK) and their constituent proteins in in vitro-matured bovine oocytes after in vitro fertilization (IVF) or after parthenogenetic activation induced by calcium ionophore A23187 alone or by theionophore followed by either 6-dimethylaminopurine (6-DMAP) or cycloheximide (CHX), Inactivation of both H1 kinase and MAPK occurred after both A23187+6-DMAP treatment and IVF; inactivation of H1 kinase preceded inactivationof MAPK, However, MAPK was inactivated much earlier in 6-DMAP-treated oocytes, Further analysis of constituent cell cycle proteins of these kinases by Western blot showed that A23187 alone could not induce changes in cdc2, cdc25, or ERK2 but induced reduction of cyclin B1, IVF and A23187+CHX induced similar changes: cyclin B1 was destroyed shortly after activation followed by accumulation of cyclin B1, phosphorylation of cdc2, and dephosphorylation of ERK2 at pronuclear formation 15 h after activation. No change in cdc25 was observed at this time. In contrast, A23187+6-DMAP treatment resultedin earlier phosphorylation of cdc2 and dephosphorylation of ERK2 at 4 h after treatment when the pronucleus formed. Moreover, accumulation of both cdc25 and cyclin B1 was detected at 15 h, Microinjection of ERK2 antibody into A23187-treated oocytes resulted in pronuclear formation. In conclusion, activation of bovine oocytes with 6-DMAP led to earlier inactivation of MAPK, while CHX induced inactivation of MAPK parallel to that following sperm-induced oocyte activation. Destruction of cyclin B is responsible for inactivation of MPF, while phosphorylation of cdc2 is likely responsible for maintaining its low activity, Inactivation of MAPK is closely associated with pronuclear development regardless of the activation protocol used.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 05:37:19