Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Use of conserved randomly amplified polymorphic DNA (RAPD) fragments and RAPD pattern for characterization of Lactobacillus fermentum in Ghanaian fermented maize dough
Autore:
Hayford, AE; Petersen, A; Vogensen, FK; Jakobsen, M;
Indirizzi:
Royal Vet & Agr Univ, Dept Dairy & Food Sci, DK-1958 Frederiksberg, Denmark Royal Vet & Agr Univ Frederiksberg Denmark DK-1958 rederiksberg, Denmark Food Res Inst, Accra, Ghana Food Res Inst Accra GhanaFood Res Inst, Accra, Ghana
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 7, volume: 65, anno: 1999,
pagine: 3213 - 3221
SICI:
0099-2240(199907)65:7<3213:UOCRAP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
D-LACTATE DEHYDROGENASE; DELBRUECKII SUBSP BULGARICUS; LACTIC-ACID BACTERIA; D-2-HYDROXYISOCAPROATE DEHYDROGENASE; LENGTH-POLYMORPHISM; PLANTARUM STRAINS; KENKEY PRODUCTION; GENE; REPRODUCIBILITY; CLASSIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Hayford, AE Royal Vet & Agr Univ, Dept Dairy & Food Sci, Rolighedsvej 30, DK-1958 Frederiksberg, Denmark Royal Vet & Agr Univ Rolighedsvej 30 Frederiksberg Denmark DK-1958
Citazione:
A.E. Hayford et al., "Use of conserved randomly amplified polymorphic DNA (RAPD) fragments and RAPD pattern for characterization of Lactobacillus fermentum in Ghanaian fermented maize dough", APPL ENVIR, 65(7), 1999, pp. 3213-3221

Abstract

The present work describes the use of randomly amplified polymorphic DNA (RAPD) for the characterization of 172 dominant Lactobacillus isolates from present and previous studies of Ghanaian maize fermentation. Heterofermentative lactobacilli dominate the fermentation flora, since approximately 85% of the isolates belong to this group, Cluster analysis of the RAPD profilesobtained showed the presence of two main clusters. Cluster 1 included Lactobacillus fermentum, whereas cluster 2 comprised the remaining Lactobacillus spp, The two distinct clusters emerged at the similarity level of <50%. All isolates in cluster 1 showed similarity in their RAPD profile to the reference strains of L. fermentum included in the study. These isolates, yielding two distinct bands of approximately 695 and 773 bp with the primers used, were divided into four subclusters, indicating that several strains are involved in the fermentation and remain dominant throughout the process. The two distinct RAPD fragments were cloned, sequenced, and used as probes inSouthern hybridization experiments. With one exception, Lactobacillus reuteri LMG 13045, the probes hybridized only to fragments of different sizes in EcoRI-digested chromosomal DNA of L. fermentum strains, thus indicating the specificity of the probes and variation within the L. fermentum isolates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:36:46