Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Serotyping and identification of Campylobacter jejuni and Campylobacter coli strains of human and animal origin using the PCR method
Autore:
Steinhauserova, I; Fojtikova, K;
Indirizzi:
Univ Vet & Pharmaceut Sci, Fac Vet Hyg & Ecol, Dept Meat Sci, Brno, Czech Republic Univ Vet & Pharmaceut Sci Brno Czech Republic Sci, Brno, Czech Republic
Titolo Testata:
ACTA VETERINARIA BRNO
fascicolo: 2, volume: 68, anno: 1999,
pagine: 149 - 154
SICI:
0001-7213(199906)68:2<149:SAIOCJ>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
POULTRY; CELLS;
Keywords:
Campylobacter jejuni coli; PCR; HS antigen; food-borne diseases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Steinhauserova, I Univ Vet & Pharmaceut Sci, Dept Meat Hyg & Technol, Palackeho 1-3, Brno 61242, Czech Republic Univ Vet & Pharmaceut Sci Palackeho 1-3 Brno Czech Republic 61242
Citazione:
I. Steinhauserova e K. Fojtikova, "Serotyping and identification of Campylobacter jejuni and Campylobacter coli strains of human and animal origin using the PCR method", ACT VET B, 68(2), 1999, pp. 149-154

Abstract

A total number of 178 strains of Campylobacter spp. from human patients suffering from diarrhea and from the intestinal content of slaughtered pigs and poultry was isolated during years 1996 to 1997. Campylobacter strains were isolated and identified by standard tests and the PCR method using fla Aand fla B, as well. Isolates originating from human patients were identified as Campylobacter jejuni (98% = 108 strains) and C. coli (2% = 2 strains). Campylobacter spp. (20) strains obtained from slaughtered pigs were identified using standard tests in 45% (9 strains) and 40% (8 strains) as C. coil and C. jejuni, respectively. Strains of Campylobacter spp. (48) isolated from slaughtered poultry were identified as C. jejuni (89% = 42 strains). Standard tests failed to identify 9 strains; 6 strains were identified as Campylobacter jejuni and C. coil using the PCR method. Serotyping using 34 antisera of the Penner's serotyping scheme was performed in 130 strains of Campylobacter jejuni isolated from poultry and human patients. This method was suitable for identification of almost 80% of C. jejuni strains. We found 10 different serotypes of human C. jejuni strains. There were serotypes (HS) 4, 1 and 9 (32%, 23% and 15%, respectively) predominating in the human population; they were followed by serotypes (HS) 10, 2 and 23 (8%, 5% and 3%,respectively). Animal strains included 7 (HS) serotypes. Like in the humanstrains, the serotype 4, found in 37% of examined isolates, was predominating. Serotypes 23 and 1 (17% and 15%, respectively) were the next most frequent ones. Comparing the obtained serotypes of human and animal strains we see that there are only 4 identical ones (i.e., 4, 1, 10, 23), which are, however, most frequently occurring in both groups of strains. The PCR methods and serotyping may facilitate and make more accurate the results of standard cultivation and in epidemiologically serious cases help in explaining the route of transmission.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 16:46:26