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Titolo:
5-hydroxytryptamine(2C) receptors on spinal neurons controlling penile erection in the rat
Autore:
Bancila, M; Verge, D; Rampin, O; Backstrom, JR; Sanders-Bush, E; McKenna, KE; Marson, L; Calas, A; Giuliano, F;
Indirizzi:
Univ Paris 06, Dept Neurobiol Signaux Intercellulaires, Inst Neurosci, CNRS,UMR 7624, F-75005 Paris, France Univ Paris 06 Paris France F-75005 CNRS,UMR 7624, F-75005 Paris, France INRA, Lab Neurobiol Fonct Vegetat, F-78352 Jouy En Josas, France INRA Jouy En Josas France F-78352 Vegetat, F-78352 Jouy En Josas, France Vanderbilt Univ, Sch Med, Dept Pharmacol, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 Pharmacol, Nashville, TN 37232 USA Vanderbilt Univ, Sch Med, Ctr Mol Neurosci, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 Neurosci, Nashville, TN 37232 USA Northwestern Univ, Sch Med, Dept Physiol, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 ept Physiol, Chicago, IL 60611 USA Fac Med Paris Sud, Grp Rech Urol, F-94270 Le Kremlin Bicetre, France Fac Med Paris Sud Le Kremlin Bicetre France F-94270 mlin Bicetre, France Univ N Carolina, Sch Med, Div Urol, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 Urol, Chapel Hill, NC 27599 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 4, volume: 92, anno: 1999,
pagine: 1523 - 1537
SICI:
0306-4522(1999)92:4<1523:5ROSNC>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; HIPPOCAMPAL PYRAMIDAL CELLS; DORSAL RAPHE NEURONS; PREGANGLIONIC NEURONS; PSEUDORABIES VIRUS; NUCLEUS PARAGIGANTOCELLULARIS; META-CHLOROPHENYLPIPERAZINE; SEROTONERGIC INNERVATION; 5-HT2C RECEPTORS; SEXUAL REFLEXES;
Keywords:
5-HT2C receptors; immunocytochemistry; rat; spinal cord; penile erection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Verge, D Univ Paris 06, Dept Neurobiol Signaux Intercellulaires, Inst Neurosci, CNRS,UMR 7624, F-75005 Paris, France Univ Paris 06 Paris France F-75005 7624, F-75005 Paris, France
Citazione:
M. Bancila et al., "5-hydroxytryptamine(2C) receptors on spinal neurons controlling penile erection in the rat", NEUROSCIENC, 92(4), 1999, pp. 1523-1537

Abstract

The localization of 5-hydroxytryptamine(2C) receptors in the lumbosacral spinal cord of the rat was investigated using selective antibodies raised against the carboxyl-terminal part of the rat receptor. The distribution of immunoperoxidase labelling at the light microscope level revealed numerous labelled neurons in the gray matter, with a higher intensity in the sacral parasympathetic nucleus, the dorsal gray commissure and particularly the motoneurons of the ventral horn. Confocal microscope analysis showed that immunostaining was mainly intracellular (motoneurons), but could also be associated with the membrane of cell bodies and dendrites. Actually, electron microscope immunogold experiments demonstrated an exclusive staining of the cis-Golgi apparatus. Following pseudo-rabies virus transsynaptic retrograde labelling from the corpus cavernosum, labelled neurons were found in the sacral parasympathetic nucleus and the dorsal gray commissure of the L6-S1 segments, All virus-labelled neurons exhibited 5-hydroxytryptamine(2C) receptor immunoreactivity. These results indicate that all parasympathetic preganglionic neurons and their related interneurons which contribute to the innervation of cavernosal tissue bear 5-hydroxytryptamine(2C) receptors. In the sacral parasympathetic nucleus, most neurons which were retrogradely-labelled from the pelvic ganglion with Fast Blue also showed 5-hydroxytrypramine(2c) receptor immunoreactivity. In the ventral horn, motoneurons retrogradely labelled from the ischiocavernosus muscle and the bulbospongiosus muscle,both of which are involved in erection and ejaculation, were also 5-hydroxytryptamine(2C) receptor-immunopositive. The supraspinal serotoninergic control of erection at the lumbosacral level therefore appears to be strongly associated with the activation of 5-hydroxytryptamine(2C) receptors, consistent with the proerectile properties of 5-hydroxytryptamine(2C) agonists. (C) 1999 IBRO. Published by Elsevier Science Ltd.

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Documento generato il 29/03/20 alle ore 15:40:58