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Titolo:
Isolation of bovine neutrophils with biomagnetic beads: comparison with standard Percoll density gradient isolation methods
Autore:
Soltys, J; Swain, SD; Sipes, KM; Nelson, LK; Hanson, AJ; Kantele, JM; Jutila, MA; Quinn, MT;
Indirizzi:
Montana State Univ, Dept Vet Mol Biol, Bozeman, MT 59717 USA Montana StateUniv Bozeman MT USA 59717 t Mol Biol, Bozeman, MT 59717 USA
Titolo Testata:
JOURNAL OF IMMUNOLOGICAL METHODS
fascicolo: 1-2, volume: 226, anno: 1999,
pagine: 71 - 84
SICI:
0022-1759(19990624)226:1-2<71:IOBNWB>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMORPHONUCLEAR LEUKOCYTES; L-SELECTIN; STAPHYLOCOCCUS-AUREUS; CHEMOTACTIC FACTORS; BURST ACTIVITY; BLOOD; IDENTIFICATION; EXPRESSION; MAC-1; SEPARATION;
Keywords:
polymorphonuclear leukocyte; bovine; chemotaxis; host defense; phagocytosis; respiratory burst; apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Quinn, MT Montana State Univ, Dept Vet Mol Biol, Bozeman, MT 59717 USA Montana State Univ Bozeman MT USA 59717 , Bozeman, MT 59717 USA
Citazione:
J. Soltys et al., "Isolation of bovine neutrophils with biomagnetic beads: comparison with standard Percoll density gradient isolation methods", J IMMUNOL M, 226(1-2), 1999, pp. 71-84

Abstract

A prerequisite for studies on bovine neutrophils is a reliable method of neutrophil isolation from blood to obtain highly purified cell populations that are functionally active. Since current techniques of neutrophil isolation fall short of these requirements, we have developed a newer and more effective technique for isolation of bovine neutrophils that utilizes biomagnetic beads coated with a monoclonal antibody that recognizes an abundant surface antigen on bovine neutrophils to purify these cells. Comparison of thepurity and viability of bovine neutrophils isolated by a conventional method (continuous Percoll density gradient) with this new method showed that neutrophils isolated with biomagnetic beads were higher in purity and had anincreased yield. In addition, cells isolated with biomagnetic beads demonstrated normal or even improved function in assays of chemotaxis, phagocytosis, degranulation, and respiratory burst activity. Finally, bovine neutrophils isolated using this method showed an overall lower level of spontaneousapoptosis, which correlates well with the high level of viability observedin the purified cell preparations. Thus, this method represents a significant advance over current methods for isolating bovine neutrophils and wouldbe widely applicable to labs studying the biochemistry and signal transduction pathways in these cells. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 04:24:10