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Titolo:
Transfer ratio in indirect UV detection in capillary zone electrophoresis - A mathematical approach
Autore:
Beckers, JL;
Indirizzi:
Eindhoven Univ Technol, Dept Chem SPO, NL-5600 MB Eindhoven, Netherlands Eindhoven Univ Technol Eindhoven Netherlands NL-5600 MB ven, Netherlands
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 844, anno: 1999,
pagine: 321 - 331
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREDICTING PEAK SYMMETRY; SYSTEM PEAKS;
Keywords:
transfer ratio; background electrolyte composition; mathematical modelling; inorganic cations; organic acids; imidazole; histidine; ethanolamine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Beckers, JL Eindhoven Univ Technol, Dept Chem SPO, POB 513, NL-5600 MB Eindhoven, Netherlands Eindhoven Univ Technol POB 513 Eindhoven Netherlands NL-5600 MB
Citazione:
J.L. Beckers, "Transfer ratio in indirect UV detection in capillary zone electrophoresis - A mathematical approach", J CHROMAT A, 844(1-2), 1999, pp. 321-331

Abstract

Generally, the transfer ratio (TR) is defined as the number of molecules of the background electrolyte (BGE) with UV absorbing properties, displaced by each analyte molecule, and this concept plays an important part in the choice of the BCE in capillary zone electrophoresis (CZE) in the indirect UVmode. Less transparent is this concept and its importance, applying BGEs with more than one co-ions, in the application of UV absorbing counterions and for the use of BGEs at low or high pH values. In this paper, the conceptof the TR is discussed for these cases and applying a mathematical model, with which all parameters in the sample zones can be calculated, the TR values are calculated for several BGEs. Hereby, different values are obtained for anionic and cationic TR values for all ionic species of the BGE. Discontinuities in the relationships of the calculated TR values versus the mobilities of the sample components indicate that system peaks are present in the electropherograms applying BGEs with two co-ions and also applying BGEs with a single co-ion at both high and low pH values. This means that in the latter cases OH- and H+ ions act as a second co-ion, whereby the mobilitiesof the system peaks generally increase for higher and lower pH values, respectively. If the mobilities of the analytes approach the mobility of a system peak, the TR values increase considerably. These phenomena are confirmed experimentally. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 06/07/20 alle ore 08:25:17