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Titolo:
Keratin filament suspensions show unique micromechanical properties
Autore:
Ma, LL; Xu, JY; Coulombe, PA; Wirtz, D;
Indirizzi:
Johns Hopkins Univ, Sch Med, Dept Biol Chem, Baltimore, MD 21205 USA JohnsHopkins Univ Baltimore MD USA 21205 l Chem, Baltimore, MD 21205 USA Johns Hopkins Univ, Sch Med, Dept Dermatol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 rmatol, Baltimore, MD 21205 USA Johns Hopkins Univ, Dept Chem Engn, Baltimore, MD 21218 USA Johns Hopkins Univ Baltimore MD USA 21218 m Engn, Baltimore, MD 21218 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 27, volume: 274, anno: 1999,
pagine: 19145 - 19151
SICI:
0021-9258(19990702)274:27<19145:KFSSUM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
WHITE SPONGE NEVUS; INTERMEDIATE FILAMENTS; MECHANICAL-PROPERTIES; TRANSGENIC MICE; ROD DOMAIN; ACTIN GELS; F-ACTIN; NETWORKS; VIMENTIN; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Coulombe, PA Johns Hopkins Univ, Sch Med, Dept Biol Chem, 725 N Wolfe St, Baltimore, MD21205 USA Johns Hopkins Univ 725 N Wolfe St Baltimore MD USA 21205 USA
Citazione:
L.L. Ma et al., "Keratin filament suspensions show unique micromechanical properties", J BIOL CHEM, 274(27), 1999, pp. 19145-19151

Abstract

All epithelial cells feature a prominent keratin intermediate filament (IF) network in their cytoplasm. Studies in transgenic mice and in patients with inherited epithelial fragility syndromes showed that a major function ofkeratin Ifs is to provide mechanical support to epithelial cell sheets. Yet the micromechanical properties of keratin Ifs themselves remain unknown. We used rheological methods to assess the properties of suspensions of epidermal type I and type II keratin Ifs and of vimentin, a type III LF polymer. We find that both types of Ifs form gels with properties akin to visco elastic solids. With increasing deformation they display strain hardening andyield relatively rapidly. Remarkably, both types of gels recover their preshear properties upon cessation of the deformation. Repeated imposition of small deformations gives rise to a progressively stiffer gel for keratin but not vimentin Ifs. The visco-elastic moduli of both gels show a weak dependence upon the frequency of the input shear stress and the concentration ofthe polymer, suggesting that both steric and nonsteric interactions between individual polymers contribute to the observed mechanical properties. In support of this, the length of individual polymers contributes only modestly to the properties of IF gels. Collectively these properties render Ifs unique among cytoskeletal polymers and have strong implications for their function in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:37:44