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Titolo:
Human p53-p51 (p53-related) fusion protein: A potent BAX transactivator
Autore:
Ikawa, S; Obinata, M; Ikawa, Y;
Indirizzi:
Tokyo Med & Dent Univ, Dept Retroviral Regulat, Div Med Res, Bunkyo Ku, Tokyo 1138519, Japan Tokyo Med & Dent Univ Tokyo Japan 1138519 unkyo Ku, Tokyo 1138519, Japan Tohoku Univ, Inst Dev Aging & Canc, Dept Cell Biol, Aoba Ku, Sendai, Miyagi 9808575, Japan Tohoku Univ Sendai Miyagi Japan 9808575 Ku, Sendai, Miyagi 9808575, Japan
Titolo Testata:
JAPANESE JOURNAL OF CANCER RESEARCH
fascicolo: 6, volume: 90, anno: 1999,
pagine: 596 - 599
SICI:
0910-5050(199906)90:6<596:HP(FPA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING; P53 HOMOLOG; GENE; P73;
Keywords:
p53-p51; chimeric construct; transactivator; gene therapy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Ikawa, Y Tokyo Med & Dent Univ, Dept Retroviral Regulat, Div Med Res, Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138519, Japan Tokyo Med & Dent Univ 1-5-45 Yushima Tokyo Japan 1138519 , Japan
Citazione:
S. Ikawa et al., "Human p53-p51 (p53-related) fusion protein: A potent BAX transactivator", JPN J CANC, 90(6), 1999, pp. 596-599

Abstract

We recently discovered human p51, a new gene structurally and functionallyrelated to human p53, This gene encodes two major splicing variants, p51A and p51B, which differ in their carboxy-terminal structure. However, p51A shows strong transactivation potential, while p51B has only weak potential. To clarify the reason for this difference, we made chimeric gene constructsexpressing fusion proteins of p53-p51A and p53-p51B, having an N-terminus of p53 and a C-terminus of p51A. or p51B, respectively, In a BAX promoter-luciferase assay using p53-deficient SAOS-2 cells, they exhibited up to 30-fold stronger transactivation potential than p53 and p51A themselves, suggesting that the C-terminus of p51B does not simply serve as a repressor We obtained similar results with p21(WAF1) promoter-reporter plasmids, These chimeras will,be valuable tools for gene therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:01:34