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Titolo:
Establishment of antigen-specific IgE transgenic mice to study pathological and immunobiological roles of IgE in vivo
Autore:
Matsuoka, K; Taya, C; Kubo, S; Toyama-Sorimachi, N; Kitamura, F; Ra, C; Yonekawa, H; Karasuyama, H;
Indirizzi:
Tokyo Metropolitan Inst Med Sci, Dept Immunol, Bunkyo Ku, Tokyo 113, JapanTokyo Metropolitan Inst Med Sci Tokyo Japan 113 kyo Ku, Tokyo 113, Japan Tokyo Metropolitan Inst Med Sci, Dept Lab Anim Sci, Bunkyo Ku, Tokyo 113, Japan Tokyo Metropolitan Inst Med Sci Tokyo Japan 113 kyo Ku, Tokyo 113, Japan Juntendo Univ, Sch Med, Dept Immunol, Tokyo 113, Japan Juntendo Univ Tokyo Japan 113 v, Sch Med, Dept Immunol, Tokyo 113, Japan
Titolo Testata:
INTERNATIONAL IMMUNOLOGY
fascicolo: 6, volume: 11, anno: 1999,
pagine: 987 - 994
SICI:
0953-8178(199906)11:6<987:EOAITM>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYPERSENSITIVITY REACTIONS; MAST-CELLS; SERUM IGE; ANTIBODIES; EXPRESSION; MECHANISM; RECEPTOR; SKIN;
Keywords:
allergen; allergy; CD23; Fc epsilon RI; immediate hypersensitivity; systemic anaphylaxis; sensitization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Karasuyama, H Tokyo Metropolitan Inst Med Sci, Dept Immunol, Bunkyo Ku, 3-18-22 Honkomagome, Tokyo 113, Japan Tokyo Metropolitan Inst Med Sci 3-18-22Honkomagome Tokyo Japan 113
Citazione:
K. Matsuoka et al., "Establishment of antigen-specific IgE transgenic mice to study pathological and immunobiological roles of IgE in vivo", INT IMMUNOL, 11(6), 1999, pp. 987-994

Abstract

We have established transgenic mice that carry the genes coding for heavy and light chains of TNP-specific IgE, They produced high titers of TNP-specific IgE (20-40 mu g/ml in serum) and their mast cells were heavily loaded with IgE. The level of Fc epsilon RI expression on their mast cells was 6-8times higher than that in non-transgenic littermates, The expression of low-affinity IgE receptor Fc epsilon RII (CD23) on splenic B cells was also 6-8 times higher in the transgenic mice. Consistent with this, substantial amounts of IgE were detected on B cells in the transgenic mice. When challenged with i,v, administration of the corresponding antigen, the transgenic mice exhibited systemic anaphylactic symptoms such as a drastic drop of bodytemperature and extravasation of administered dye. Biphasic (immediate anddelayed) ear swelling response was also elicited in a TNP-specific manner by epicutaneous antigen challenge without any prior sensitization. Thus, IgE produced in the transgenic mice was found to be biologically active to induce both local and systemic allergic reactions in vivo upon the challenge of the corresponding antigen. Taken together, the antigen-specific IgE transgenic mice established for the first time in this study appear to provide an attractive model system to study the pathological roles of IgE in acute and chronic phases of allergic inflammation as well as their immunobiological roles in vivo. They may also be useful to develop novel therapeutic strategies for atopic disorders.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/21 alle ore 02:34:14