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Titolo:
Human cord blood CD34(+)CD38(-) cell transduction via lentivirus-based gene transfer vectors
Autore:
Evans, JT; Kelly, PF; ONeill, E; Garcia, JV;
Indirizzi:
St Jude Childrens Res Hosp, Dept Virol & Mol Biol, Memphis, TN 38105 USA St Jude Childrens Res Hosp Memphis TN USA 38105 ol, Memphis, TN 38105 USA St Jude Childrens Res Hosp, Dept Hematol, Memphis, TN 38105 USA St Jude Childrens Res Hosp Memphis TN USA 38105 ol, Memphis, TN 38105 USA Univ Tennessee, Dept Pathol, Memphis, TN 38103 USA Univ Tennessee MemphisTN USA 38103 e, Dept Pathol, Memphis, TN 38103 USA
Titolo Testata:
HUMAN GENE THERAPY
fascicolo: 9, volume: 10, anno: 1999,
pagine: 1479 - 1489
SICI:
1043-0342(19990610)10:9<1479:HCBCCT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; HEMATOPOIETIC STEM-CELLS; SCID-REPOPULATING CELLS; NONDIVIDING HUMAN-CELLS; MURINE LEUKEMIA-VIRUS; BONE-MARROW; IN-VIVO; RETROVIRAL VECTOR; HUMAN-LYMPHOCYTES; HIGHLY EFFICIENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Garcia, JV Univ Texas, SW Med Ctr, Dept Internal Med, Div Infect Dis, 5323Harry Hines Blvd, Dallas, TX 75235 USA Univ Texas 5323 Harry Hines Blvd Dallas TX USA 75235 75235 USA
Citazione:
J.T. Evans et al., "Human cord blood CD34(+)CD38(-) cell transduction via lentivirus-based gene transfer vectors", HUM GENE TH, 10(9), 1999, pp. 1479-1489

Abstract

The efficient transfer and sustained expression of a transgene in human hematopoietic cells with in vivo repopulating potential would provide a significant advancement in the development of protocols for the treatment of hematopoietic diseases. Recent advances in the ability to purify and culture hematopoietic cells,vith the CD34(+)CD38(-) phenotype and with in vivo repopulating potential from human umbilical cord blood provide a direct means oftesting the ability of transfer vectors to transduce these cells. Here we demonstrate the efficient transduction and expression of enhanced green fluorescent protein (EGFP) in human umbilical cord-derived CD34(+)CD38(-) cells, without prestimulation, using a lentivirus-based gene transfer system. Transduced CD34(+)CD38(-) cells cultured in serum-free medium supplemented with SCF, Flt-3, IL-3, and IL-6 maintained their surface phenotype for 5 days and expressed readily detectable levels of the transgene. The average transduction efficiency of the CD34+CD38- cells was 59 +/- 7% as determined byflow cytometry, Erythroid and myeloid colonies derived from transduced CD34+CD38- cells were EGFP positive at a high frequency (66 +/- 9%). In contrast, a murine leukemia virus-based vector transduced the CD34(+)CD38(-) cells at a low frequency (<4%), These results demonstrate the utility of lentiviral-based gene transfer vectors in the transduction of primitive human hematopoietic CD34(+)CD38(-) cells.

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Documento generato il 04/12/20 alle ore 19:28:00