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Titolo:
Evaluation of the human choroidal melanoma rabbit model for studying microcirculation patterns with confocal ICG and histology
Autore:
Mueller, AJ; Folberg, R; Freeman, WR; Bartsch, DU; Bergeron-Lynn, G; Mehaffey, MG; Kan-Mitchell, J; Huang, XQ; Jian, G; Avila, C; Taskintuna, I; Cheng, LY; Wang, J;
Indirizzi:
Univ Calif San Diego, Shiley Eye Ctr, Dept Ophthalmol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 halmol, La Jolla, CA 92093 USA Univ Munich, Eyeclin, D-80336 Munich, Germany Univ Munich Munich GermanyD-80336 ich, Eyeclin, D-80336 Munich, Germany Univ Iowa, Dept Ophthalmol & Visual Sci, Med Res Ctr 100, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 Med Res Ctr 100, Iowa City, IA 52242 USA Univ Iowa, Dept Pathol, Med Res Ctr 100, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 Med Res Ctr 100, Iowa City, IA 52242 USA Univ Calif San Diego, Ctr Canc, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 r Canc, La Jolla, CA 92093 USA
Titolo Testata:
EXPERIMENTAL EYE RESEARCH
fascicolo: 6, volume: 68, anno: 1999,
pagine: 671 - 678
SICI:
0014-4835(199906)68:6<671:EOTHCM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CILIARY BODY MELANOMAS; HUMAN UVEAL MELANOMA; INDOCYANINE GREEN; LASER OPHTHALMOSCOPY; PROGNOSTIC VALUE; LARGEST NUCLEOLI; HETEROTRANSPLANTATION; ARCHITECTURE; ANGIOGRAPHY; INJECTION;
Keywords:
animal model; human choroidal melanoma; indocyanine green angiography; microcirculation pattern; rabbit;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Freeman, WR Univ Calif San Diego, Shiley Eye Ctr, Dept Ophthalmol, 9415 Campus Point Dr,Ste 218, La Jolla, CA 92093 USA Univ Calif San Diego 9415 Campus Point Dr,Ste 218 La Jolla CA USA 92093
Citazione:
A.J. Mueller et al., "Evaluation of the human choroidal melanoma rabbit model for studying microcirculation patterns with confocal ICG and histology", EXP EYE RES, 68(6), 1999, pp. 671-678

Abstract

The aim of this study was to develop consistently focal elevated choroidalmasses of human choroidal melanoma in immunosuppressed rabbits and to correlate the visualization of prognostically significant microcirculation patterns from confocal indocyanine green angiography with histologic microcirculation patterns. A human choroidal melanoma cell line (OCM1) was implanted in the choroid of 40 rabbit eyes using three different techniques: transscleral choroidal injection of a cell suspension, injection of a cell suspension in a surgically induced cyclodialysis cleft, and implantation of solid tumor fragments in a surgically induced cyclodialysis cleft. The rabbits were immunosuppressed with daily injections of Cyclosporin A to prevent host versus graft reaction. The eyes were studied weekly with indirect ophthalmoscopy and fundus photography to monitor tumor growth and indocyanine green angiography using a confocal scanning laser ophthalmoscope to identify microcirculation patterns in vivo and correlate these findings with the histologic demonstration of tumor microcirculation patterns. A tumor mass was identified by indirect ophthalmoscopy in 16 of the 40 implanted rabbit eyes (40 %). Each of these tumors was confirmed histologically to represent a focal elevated choroidal mass. All 16 elevated choroidal masses grow in eyes in which solid tumor fragments were implanted. In total, a melanoma was identified histologically in 28 of the implanted 40 eyes (70%). In addition to the16 eyes where the melanoma appeared as a focal elevated choroidal mass, 4 eyes contained a focal elevated mass in the sclera and 8 eyes contained a flat choroidal tumor. Histologically, microcirculation patterns were identified only in the 16 eyes with focal elevated choroidal masses. Confocal indocyanine green angiography imaged microcirculation patterns in 13 of these 16 eyes (81%). The surgical implantation of small solid fragments of human choroidal melanoma in immunosuppressed rabbit eyes provides the best method to consistently obtain focal elevated choroidal masses. These focal elevated choroidal masses resemble booth the localization and the growth pattern of choroidal melanomas in humans. In addition, they also contain microcirculation patterns similar to those seen in humans that are detectable with confocal indocyanine green angiography. The use of indocyanine green angiography with this animal model may be especially useful in designing and evaluating anti-microcirculation treatments directed at uveal melanoma. (C) 1999 Academic Press.

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Documento generato il 01/12/20 alle ore 00:32:48