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Titolo:
High-resolution electrophoretic analysis of rat parotid salivary proteins
Autore:
Williams, KM; Ekstrom, J; Marshall, T;
Indirizzi:
Univ Sunderland, Sch Hlth Sci, Analyt Biochem Res Grp, Sunderland SR1 3RD,England Univ Sunderland Sunderland England SR1 3RD p, Sunderland SR1 3RD,England Univ Gothenburg, Dept Pharmacol, Gothenburg, Sweden Univ Gothenburg Gothenburg Sweden g, Dept Pharmacol, Gothenburg, Sweden
Titolo Testata:
ELECTROPHORESIS
fascicolo: 7, volume: 20, anno: 1999,
pagine: 1373 - 1381
SICI:
0173-0835(199906)20:7<1373:HEAORP>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROLINE-RICH PROTEINS; PARASYMPATHETIC NERVE-STIMULATION; GENE-RELATED PEPTIDE; GLAND FUNCTION; CARIES DEVELOPMENT; SUBSTANCE-P; LIQUID DIET; ISOPROTERENOL; ATROPINE; PROPRANOLOL;
Keywords:
rat parotid saliva; salivary proteins; two-dimensioal electrophoresis; silver staining; sodium dodecyl sulfate-polyacrylamide gel electrophoresis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Marshall, T Univ Sunderland, Sch Hlth Sci, Analyt Biochem Res Grp, Sunderland SR1 3RD,England Univ Sunderland Sunderland England SR1 3RD d SR1 3RD,England
Citazione:
K.M. Williams et al., "High-resolution electrophoretic analysis of rat parotid salivary proteins", ELECTROPHOR, 20(7), 1999, pp. 1373-1381

Abstract

Silver staining and high-resolution electrophoretic methods have been usedto compare the protein composition of rat parotid saliva evoked in response to (i) parasympathetic stimulation (including the nonadrenergic, noncholinergic, atropine-associated secretion), (ii) sympathetic stimulation, or (iii) the infusion of neuropeptides with secretagogue activity (substance P, calcitonin gene-related peptide, neuropeptide Y, or vasoactive intestinal peptide). The different stimuli influenced the protein concentration and flow rate of the evoked secretion but had little effect upon the protein composition of the saliva. In contrast to earlier studies using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Coomassie blue staining, the combination of silver staining and two-dimensional electrophoresis (2-DE) revealed many newly detected proteins. The results indicate that the protein composition of rat parotid saliva is more complex than previously reported but is unaffected by the mode of stimulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 02:47:35