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Titolo:
Decapping of stabilized, polyadenylated mRNA in yeast pab1 mutants
Autore:
Morrissey, JP; Deardorff, JA; Hebron, C; Sachs, AB;
Indirizzi:
Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 ll Biol, Berkeley, CA 94720 USA Johnandnes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, Engl John Innes Ctr Plant Sci Res Norwich Norfolk England NR4 7UH orfolk, Engl
Titolo Testata:
YEAST
fascicolo: 8, volume: 15, anno: 1999,
pagine: 687 - 702
SICI:
0749-503X(19990615)15:8<687:DOSPMI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLY(A) BINDING-PROTEIN; UNSTABLE MESSENGER-RNAS; ACTIVATORS IN-VIVO; SACCHAROMYCES-CEREVISIAE; TRANSLATION INITIATION; POLY(A)-BINDING PROTEIN; TAIL; TURNOVER; DOMAINS; DEADENYLATION;
Keywords:
mRNA degradation; mRNA processing; PAB1; poly(A); yeast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Sachs, AB Univ720lif Berkeley, Dept Mol & Cell Biol, 401 Barker Hall, Berkeley, CA 94 Univ Calif Berkeley 401 Barker Hall Berkeley CA USA 94720 CA 94
Citazione:
J.P. Morrissey et al., "Decapping of stabilized, polyadenylated mRNA in yeast pab1 mutants", YEAST, 15(8), 1999, pp. 687-702

Abstract

Interaction of the poly(A) binding protein, Pab1p, with mRNA plays an important role in gene expression. This work describes an analysis of pab1 mutants in Saccharomyces cerevisiae. Yeast pab1 mutants were found to be sensitive to elevated concentrations of copper (Cu) and 3-aminotriazole (3-AT) inthe growth medium. This phenotype arises because these pab1 mutants underaccumulate mRNA, including the CUP1 and HIS3 mRNAs, the products of which are required for Cu and 3-AT resistance, respectively. To determine the causeof the mRNA underaccumulation, mRNA turnover and production were examined in the pab1-53 mutant. It was found that although the pattern of mRNA decaywas altered, and substantial decapping of polyadenylated mRNA could be detected, mRNA was not destabilized in this strain. It was also found that thepab1 mutant was impaired in the production of mRNA. These data show that the decreased level of mRNA in the pab1-53 mutant arises from poor production, and they suggest that yeast Pab1p is involved in an aspect of nuclear mRNA metabolism. They also indicate that deadenylation can be uncoupled from decapping without significant changes in an mRNA's stability, and that thisuncoupling can be tolerated by yeast. Copyright (C) 1999 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 07:04:01