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Titolo:
CELLULAR-DISTRIBUTION OF ISOFORMS OF PROTEIN-KINASE-C (PKC) IN PANCREATIC ACINI
Autore:
BASTANI B; YANG LY; BALDASSARE JJ; POLLO DA; GARDNER JD;
Indirizzi:
ST LOUIS UNIV,CTR HLTH SCI,DEPT INTERNAL MED,1402 S GRAND BLVD ST LOUIS MO 63104 ST LOUIS UNIV,CTR HLTH SCI,DEPT INTERNAL MED ST LOUIS MO 63104 ST LOUIS UNIV,CTR HLTH SCI,DIV NEPHROL ST LOUIS MO 63104
Titolo Testata:
Biochimica et biophysica acta. Molecular cell research
fascicolo: 3, volume: 1269, anno: 1995,
pagine: 307 - 315
SICI:
0167-4889(1995)1269:3<307:COIOP(>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-SPECIES ANALYSIS; EPIDERMAL GROWTH-FACTOR; ALPHA-THROMBIN; CULTURED FIBROBLASTS; HUMAN PLATELETS; TRANSLOCATION; ZETA; ISOZYMES; FAMILY; CELLS;
Keywords:
DIACYLGLYCEROL; TRANSLOCATION PKC; CHOLECYSTOKININ; PROTEIN KINASE C; TETRADECANOYL PHORBOL ACETATE; L-364,718;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
B. Bastani et al., "CELLULAR-DISTRIBUTION OF ISOFORMS OF PROTEIN-KINASE-C (PKC) IN PANCREATIC ACINI", Biochimica et biophysica acta. Molecular cell research, 1269(3), 1995, pp. 307-315

Abstract

As in a previous study (Biochim. Biophys. Acta 1224 (1994) 127-138), we used quantitative immunoblot analysis and found that rat pancreaticacini possess four different isoforms of PKC-alpha, delta, epsilon and zeta. The phorbol ester 12-O-tetradecanoyl phorbol 13-acetate (TPA) caused translocation of each isoform from the cytosol to the membrane fraction. CCK-8 increased diacylglycerol (DAG) and caused translocation of PKC-delta and PKC-epsilon but not that of PKC-alpha or PKC-zeta. L-364,718, a CCK receptor antagonist, prevented as well as reversed the effects of CCK-8 on DAG and on translocation of PKC-delta and PKC-epsilon. To explore the possibility that different isoforms of PKC mighthave different distributions in rat pancreas, we used immunocytochemistry to determine the cellular distribution of different isoforms of PKC in intact pancreas as well as pancreatic acini. In intact pancreas,PKC-alpha and PKC-delta were detected in islet cells but not in duct or acinar cells. PKC-epsilon was detected in the apical region of acinar cells and PKC-zeta was detected over the luminal surfaces of acinarcells and the ductules that extend from the acinus. Neither PKC-epsilon nor PKC-zeta was detected in islets. In pancreatic acini PKC-alpha and PKC-delta were detected in islets or fragments of islets that contaminated the preparation but were not detected in acinar cells. PKC-epsilon was detected in the apical region of acinar cells and adding 1 mu M TPA or 1 mu M CCK-8 accentuated the immunostaining but did not alter its cellular distribution. L-364,718 reversed the changes in immunostaining caused by CCK-8. PKC-zeta was detected over the luminal surface of the acinar cells. TPA, but not CCK-8 or CCK-8 followed by L-364,718, increased the number of acini that showed staining of the luminalsurfaces of acinar cells. Thus, the present results demonstrate that different isoforms of PKC are distributed differently in rat pancreas and that the different patterns of distribution can explain, at least in part, the different responses to CCK-8.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:40:21