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Titolo:
Development of a septicaemic murine model allowing selection of virulent mutants of Streptococcus pneumoniae
Autore:
Amory-Rivier, C; Rieux, V; Azoulay-Dupuis, E; Carbon, C; Trombe, MC;
Indirizzi:
Hop Bichat Claude Bernard, INSERM, CRI 9802, F-75877 Paris 18, France Hop Bichat Claude Bernard Paris France 18 9802, F-75877 Paris 18, France Univ Toulouse 3, CHU Rangueil, F-31054 Toulouse, France Univ Toulouse 3 Toulouse France F-31054 ngueil, F-31054 Toulouse, France
Titolo Testata:
PATHOLOGIE BIOLOGIE
fascicolo: 5, volume: 47, anno: 1999,
pagine: 519 - 525
SICI:
0369-8114(199905)47:5<519:DOASMM>2.0.ZU;2-B
Fonte:
ISI
Lingua:
FRE
Soggetto:
GRAM-POSITIVE BACTERIA; PNEUMOCOCCAL VACCINE; ESCHERICHIA-COLI; SHUTTLE VECTORS; GENES; IDENTIFICATION; RESISTANCE; PENICILLIN; EXPRESSION; AUTOLYSIS;
Keywords:
Streptococcus pneumoniae; septicaemic murin model; positive screening; bacterial transformation; virulent mutant;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Amory-Rivier, C Hopard,hat Claude Bernard, INSERM, CRI 9802, Batiment U13,46 Rue Henri Huch Hop Bichat Claude Bernard Batiment U13,46 Rue Henri Huchard Paris France 18
Citazione:
C. Amory-Rivier et al., "Development of a septicaemic murine model allowing selection of virulent mutants of Streptococcus pneumoniae", PATH BIOL, 47(5), 1999, pp. 519-525

Abstract

Genetic construction of virulence deficient mutant is a strategy to analyse virulence genes of Streptococcus pneumoniae and was used to virulence factors as capsule, pneumolysin, autolysin and PspA. We perform a model allowing the in vivo positive selection of virulent S. pneumoniae mutants. Mice which are the most susceptible animals to pneumococcal infection, offer the best model for screening virulent S. pneumoniae. Indeed after intraperitoneal injection of bacterial mix which was composed to a lot of avirulent bacteria (6 log(10) CFU per mouse) (V1015 strain, DL50 = 7.05) and Pew virulentpneumococci (1 to 2 log(10) CFU per mouse) (P4241 strain, DL50 < 1), mice cleared all avirulent bacteria but not virulent pneumococci. Thus, mice dead in 3 to 4 days with septicaemia and positive hemoculture contained only virulent strain. This model was validated by in vivo selection of a virulentmutant (V1042, DL50 = 4.1) which was obtained after transformation of avirulent strain V1015 with the genomic fragment of virulent strain P4241. Our model of screening was the only one allowing detection of virulent S. pneumoniae mutants. This new genetic strategy which consisted in gene addition and used mouse as selection agent, could be used to discover new virulence genes required to in vivo bacterial development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 15:55:42