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Titolo:
Identification of Heterodera avenae group species by morphometrics and rDNA-RFLPs
Autore:
Subbotin, SA; Waeyenberge, L; Molokanova, IA; Moens, M;
Indirizzi:
Russian Acad Sci, Inst Parasitol, Moscow 117071, Russia Russian Acad Sci Moscow Russia 117071 t Parasitol, Moscow 117071, Russia Agr Res Ctr, B-9820 Merelbeke, Belgium Agr Res Ctr Merelbeke Belgium B-9820 Res Ctr, B-9820 Merelbeke, Belgium
Titolo Testata:
NEMATOLOGY
, volume: 1, anno: 1999,
parte:, 2
pagine: 195 - 207
SICI:
1388-5545(199904)1:<195:IOHAGS>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
FRAGMENT-LENGTH-POLYMORPHISM; TRANSCRIBED SPACER REGION; CEREAL CYST NEMATODES; SP-N NEMATODA; GEL-ELECTROPHORESIS; PROTEIN-PATTERNS; NEW-ZEALAND; COMPLEX; GRASS; DNA;
Keywords:
canonical discriminant analysis; cyst nematodes; Heterodera avenae; H-filipjevi; identification; ITS-rDNA; morphometrics; RFLP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Subbotin, SA Russiansiaad Sci, Inst Parasitol, Leninskii Prospect 33, Moscow 117071, Rus Russian Acad Sci Leninskii Prospect 33 Moscow Russia 117071
Citazione:
S.A. Subbotin et al., "Identification of Heterodera avenae group species by morphometrics and rDNA-RFLPs", NEMATOLOGY, 1, 1999, pp. 195-207

Abstract

Canonical discriminant analysis of four morphometric characters of juveniles and restriction enzymes analysis of ribosomal DNA sequences were used todistinguish Heterodera arenaria, H. aucklandica, H. avenae, H. filipjevi, H. hordecalis, H. iri, H. latipons, H. litoralis, H. schachtii and an undescribed species from grasslands. The results of unweighted pair group cluster analysis showed that H. avenae populations formed three groups and H. filipjevi two groups at the 80% level of similarity. Intraspecific polymorphism was revealed by rDNA-RFLP studies and two types of ITS regions within H. avenae populations can be distinguished. The pattern of restriction bands obtained with BsuRI, PstI and TaqI clearly distinguished populations of H. filipjevi from other species of the H. avenae group. Further enzymes and their combinations distinguished the other species. There are no enzymes whichdifferentiate European populations of H. avenae from H. arenaria. Morphometrics, restriction endonuclease cleavage maps of ITS regions and a dendrogram of putative phylogenetic relations of several cyst-forming nematode species are given.

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Documento generato il 02/12/20 alle ore 08:18:15