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Titolo:
Regulation of Na+-K+-2Cl(-) cotransport in turkey red cells: the role of oxygen tension and protein phosphorylation
Autore:
Muzyamba, MC; Cossins, AR; Gibson, JS;
Indirizzi:
UnivdLiverpool, Dept Vet Preclin Sci, Liverpool L69 3BX, Merseyside, Englan Univ Liverpool Liverpool Merseyside England L69 3BX X, Merseyside, Englan Univ Liverpool, Sch Biol Sci, Liverpool L69 3BX, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 3BX , Merseyside, England
Titolo Testata:
JOURNAL OF PHYSIOLOGY-LONDON
fascicolo: 2, volume: 517, anno: 1999,
pagine: 421 - 429
SICI:
0022-3751(19990601)517:2<421:RONCIT>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
K-CL COTRANSPORT; CARP CYPRINUS-CARPIO; BLOOD-CELLS; ADRENERGIC RESPONSES; OKADAIC ACID; CALYCULIN-A; VOLUME; TRANSPORT; ERYTHROCYTES; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Gibson, JS UnivdLiverpool, Dept Vet Preclin Sci, Liverpool L69 3BX, Merseyside, Englan Univ Liverpool Liverpool Merseyside England L69 3BX de, Englan
Citazione:
M.C. Muzyamba et al., "Regulation of Na+-K+-2Cl(-) cotransport in turkey red cells: the role of oxygen tension and protein phosphorylation", J PHYSL LON, 517(2), 1999, pp. 421-429

Abstract

1. Na+-K+-2Cl(-) cotransport (NKCC) was studied in turkey red cells using Na+ dependence or bumetanide sensitivity of Rb-86(+) influx to monitor activity of the transporter.2. Deoxygenation was the major physiological stimulus for NKCC activity: oxygen tensions (P-O2) over the physiological range modulated the transporter, with a P-O2 for half-maximal activation of about 41 mmHg: (n = 3). In air, activity of NKCC was also stimulated by shrinkage and isoproteronol (isoprenaline, 5 mu M). By contrast, in deoxygenated cells, although the transporter activity was markedly elevated, it was no longer sensitive to volume or P-adrenergic stimulation.3. Calyculin A, a protein phosphatase inhibitor, stimulated cotransport with a lag of about 5 min. N-Ethylmaleimide (NEM) inhibited cotransport and also blocked the stimulatory effect of calyculin A if administered before calyculin A. Stimulation by calyculin a and deoxygenation were not additive. Staurosporine (2 mu M) inhibited deoxygenated-stimulated K+ influxes, but not those stimulated by calyculin A. NEM added during calyculin A stimulation, i.e. during the 5 min lag, caused transport activity to be clamped at levels intermediate between maximal (calyculin A alone) and control. Cells treated with calyculin A alone or with calyculin A followed by NEM were no longer sensitive to volume, isoproteronol or P-O2.4. The results have characterized the interaction between deoxygenation and other stimuli of NKCC activity. They have also shown that it is possible to manipulate the transporter in a reciprocal way to that shown previously for K+-Cl- cotransport.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 12:37:34