Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Empty synaptic vesicles recycle and undergo exocytosis at vesamicol-treated motor nerve terminals
Autore:
Parsons, RL; Calupca, MA; Merriam, LA; Prior, C;
Indirizzi:
Univ Vermont, Coll Med, Dept Anat & Neurobiol, Burlington, VT 05405 USA Univ Vermont Burlington VT USA 05405 Neurobiol, Burlington, VT 05405 USA Univasgowthclyde, Dept Physiol & Pharmacol, Strathclyde Inst Biomed Sci, Gl Univ Strathclyde Glasgow Lanark Scotland G4 0NR clyde Inst Biomed Sci, Gl
Titolo Testata:
JOURNAL OF NEUROPHYSIOLOGY
fascicolo: 6, volume: 81, anno: 1999,
pagine: 2696 - 2700
SICI:
0022-3077(199906)81:6<2696:ESVRAU>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
FROG NEUROMUSCULAR-JUNCTION; ACETYLCHOLINE TRANSPORT; TRANSMITTER RELEASE; SNAKE TWITCH; ENDOCYTOSIS; CURRENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Parsons, RL Univ,Vermont, Coll Med, Dept Anat & Neurobiol, C 427 Given Bldg, Burlington Univ Vermont C 427 Given Bldg Burlington VT USA 05405 rlington
Citazione:
R.L. Parsons et al., "Empty synaptic vesicles recycle and undergo exocytosis at vesamicol-treated motor nerve terminals", J NEUROPHYS, 81(6), 1999, pp. 2696-2700

Abstract

Empty synaptic vesicles recycle and undergo exocytosis at vesamicol-treated motor nerve terminals. J. Neurophysiol,. 81: 2696-2700, 1999. We investigated whether recycled cholinergic synaptic vesicles, which were not refilled with ACh, would join other synaptic vesicles in the readily releasable store near active zones, dock, and continue to undergo exocytosis during prolonged stimulation. Snake nerve-muscle preparations were treated with 5 mu Mvesamicol to inhibit the vesicular ACh transporter and then were exposed to an elevated potassium solution, 35 mM potassium propionate (35 KP), to release all preformed quanta of ACh. At vesamicol-treated endplates, miniature endplate current (MEPC) frequency increased initially from 0.4 to >300 s(-1) in 35 KP but then declined to <1 s(-1) by 90 min. The decrease in frequency was not accompanied by a decrease in MEPC average amplitude, Nerve terminals accumulated the activity-dependent dye FM1-43 when exposed to the dye for the final 6 min of a 120-min exposure to 35 KP. Thus synaptic membrane endocytosis continued at a high rate, although MEPCs occurred infrequently. After a 120-min exposure in 35 KP, nerve terminals accumulated FM1-43 and then destained, confirming that exocytosis also still occurred at a high rate. These results demonstrate that recycled cholinergic synaptic vesiclesthat were not refilled with ACh continued to dock and undergo exocytosis after membrane retrieval. Thus transport of ACh into recycled cholinergic vesicles is not a requirement for repeated cycles of exocytosis and retrievalof synaptic vesicle membrane during prolonged stimulation of motor nerve terminals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 00:53:47