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Titolo:
ATP-Stimulated Ca2+ influx and phospholipase D activities of a rat brain-derived type-7, astrocyte cell line, RBA-2, are mediated through P2x(7) receptors
Autore:
Sun, SH; Lin, LB; Hung, AC; Kuo, JS;
Indirizzi:
Natl Yang Ming Univ, Inst Neurosci, Taipei 112, Taiwan Natl Yang Ming Univ Taipei Taiwan 112 Inst Neurosci, Taipei 112, Taiwan Taichung Vet Gen Hosp, Taichung, Taiwan Taichung Vet Gen Hosp Taichung Taiwan ng Vet Gen Hosp, Taichung, Taiwan
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 1, volume: 73, anno: 1999,
pagine: 334 - 343
SICI:
0022-3042(199907)73:1<334:ACIAPD>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; P(2Z) PURINERGIC RECEPTORS; CALCIUM MOBILIZATION; EXTRACELLULAR ATP; HUMAN-LYMPHOCYTES; D ACTIVATION; INTRACELLULAR CALCIUM; CEREBELLAR ASTROCYTES; BAC1.2F5 MACROPHAGES; GLUTAMINE-SYNTHETASE;
Keywords:
ATP; 2 '- and 3 '-O-(4-benzoylbenzoyl)adenosine-5 '-triphosphate; Ca2+ influx; phospholipase D; P2X(7) receptor; type-2 astrocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Sun, SH NatlnYang Ming Univ, Inst Neurosci, 155,Sect 2,Li Non St, Taipei 112, Taiwa Natl Yang Ming Univ 155,Sect 2,Li Non St Taipei Taiwan 112 Taiwa
Citazione:
S.H. Sun et al., "ATP-Stimulated Ca2+ influx and phospholipase D activities of a rat brain-derived type-7, astrocyte cell line, RBA-2, are mediated through P2x(7) receptors", J NEUROCHEM, 73(1), 1999, pp. 334-343

Abstract

This study characterizes and examines the P2 receptor-mediated signal transduction pathway of a rat brain-derived type 2 astrocyte cell line. RBA-2. AIP induced Ca2+ influx and activated phospholipase D (PLD). The ATP-stimulated Ca2+ influx was inhibited by pretreating cells with P2 receptor antagonist, pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS), in a concentration-dependent manner. The agonist 2'- and 3'-O-(4-benzoylbenzoyl)adenosine 5'-triphosphate (BzATP) stimulated the largest increases in intracellular Ca2+ concentrations ([Ca2+](i)); ATP, 2-methylthioadenosine triphosphate tetrasodium, and ATP gamma S were much less effective, whereas UTP, ADP, alpha,beta-methylene-ATP, and beta,gamma-methylene-ATP were ineffective. Furthermore, removal of extracellular Mg2+ enhanced the ATP- and BzATP-stimulated increases in [Ca2+](i). BzATP stimulated PLD in a concentration- and time-dependent manner that could be abolished by removal of extracellularCa2+ and was inhibited by suramin, PPADS, and oxidized ATP. In addition, PLD activities were activated by the Ca2+ mobilization agent, ionomycin, in an extracellular Ca2+ concentration-dependent manner. Both staurosporine and prolonged phorbol ester treatment inhibited BzATP-stimulated PLD activity. Taken together, these data indicate that activation of the P2X(7) receptors induces Ca2+ influx and stimulates a Ca2+-dependent PLD in RBA-2 astrocytes. Furthermore, protein kinase C regulates this PLD.

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Documento generato il 04/12/20 alle ore 19:52:58