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Titolo:
Incidence of hepatocellular carcinoma in transgenic mice expressing the hepatitis B virus X-protein
Autore:
Yu, DY; Moon, HB; Son, JK; Jeong, S; Yu, SL; Yoon, H; Han, YM; Lee, CS; Park, JS; Lee, CH; Hyun, BH; Murakami, S; Lee, KK;
Indirizzi:
Korea0,es Inst Biosci & Biotechnol, Anim Mol Physiol Res Unit, Taejon 30560 Korea Res Inst Biosci & Biotechnol Taejon South Korea 305600 aejon 30560 Wonkwang Univ, Iksan, South Korea Wonkwang Univ Iksan South KoreaWonkwang Univ, Iksan, South Korea Kanazawa Univ, Canc Res Inst, Kanazawa, Ishikawa 920, Japan Kanazawa UnivKanazawa Ishikawa Japan 920 , Kanazawa, Ishikawa 920, Japan
Titolo Testata:
JOURNAL OF HEPATOLOGY
fascicolo: 1, volume: 31, anno: 1999,
pagine: 123 - 132
SICI:
0168-8278(199907)31:1<123:IOHCIT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-PRODUCT; HBX GENE; IN-VIVO; HEPATOCARCINOGENESIS; LIVER; ACTIVATION; DNA; TRANSACTIVATOR; PROTOONCOGENE; ANTIGEN;
Keywords:
HBV X-gene; hepatic tumors; proliferating cell nuclear antigen; transgenic mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Yu, DY Koreajons Inst Biosci & Biotechnol, Anim Mol Physiol Res Unit, POB 115, Tae Korea Res Inst Biosci & Biotechnol POB 115 Taejon South Korea 305600
Citazione:
D.Y. Yu et al., "Incidence of hepatocellular carcinoma in transgenic mice expressing the hepatitis B virus X-protein", J HEPATOL, 31(1), 1999, pp. 123-132

Abstract

Background/Aims: Chronic infection with hepatitis B virus is a high-risk factor for hepatocellular carcinoma in humans, The HBV X-protein, a multi-functional viral regulator, has been suspected to play a positive role in hepatocarcinogenesis, as demonstrated by the high incidence of hepatocellular carcinoma in HBx-expressing transgenic mice, although it is still controversial. The aim of this study was to generate transgenic mice expressing the HBV X-gene under authentic promoter control and to test whether the gene products can cause hepatic tumors. Methods: Three transgenic mouse lines were generated by microinjecting theX-gene construct into hybrid (C57BL/6xDBA) eggs. Gene expression was tested by protein and mRNA analyses. During an observation period of 18 months, mice were sacrificed and organs subjected to histologic examinations. Results: Grossly defined hepatocellular carcinomas reproducibly were observed in mice expressing the X-protein, which were investigated through six generations from the age of 11 to 18 months. Among 14 transgenic mice investigated from the age of 11 to 18 months, 12 were found to have hepatocellular carcinoma, grossly or microscopically. The lesion of the hepatocellular carcinoma disclosed a significant increase in the proliferating cell nuclearantigen in the nuclei,Conclusion: The incidence of hepatocellular carcinoma (86%) in our HBV X transgenic mice may be highly significant, since, except for one case, HBV X-gene transgenic mice produced in other laboratories did not develop liver tumor or any other pathologic phenomena.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 14:11:20