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Titolo:
PCR DETECTION OF HUMAN PAPILLOMAVIRUS - COMPARISON BETWEEN MY09 MY11 AND GP5+/GP6+ PRIMER SYSTEMS/
Autore:
QU WM; JIANG G; CRUZ Y; CHANG CJ; HO GYF; KLEIN RS; BURK RD;
Indirizzi:
YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT IMMUNOL & MICROBIOL,ULLMANN BLDG,ROOM 515 BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT IMMUNOL & MICROBIOL BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT PEDIAT BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT EPIDEMIOL & SOCIAL MED BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT MED BRONX NY 10461
Titolo Testata:
Journal of clinical microbiology
fascicolo: 6, volume: 35, anno: 1997,
pagine: 1304 - 1310
SICI:
0095-1137(1997)35:6<1304:PDOHP->2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; CYTOLOGICALLY NORMAL WOMEN; CERVICAL NEOPLASIA; BLOT HYBRIDIZATION; RISK-FACTORS; INFECTION; DETERMINANTS; GENOTYPES; HPV; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
W.M. Qu et al., "PCR DETECTION OF HUMAN PAPILLOMAVIRUS - COMPARISON BETWEEN MY09 MY11 AND GP5+/GP6+ PRIMER SYSTEMS/", Journal of clinical microbiology, 35(6), 1997, pp. 1304-1310

Abstract

Human papillomavirus (HPV) is an etiologic agent of cervical cancer and is the most common sexually transmitted disease in women, PCR amplification of HPV genomes is the most sensitive method for the detectionof cervicovaginal HPV, We have compared the two most commonly used PCR primer sets, My09/MY11 (MY-PCR) and GP5+/GP6+ (GPC-PCR), for the detection of HPV DNA in cervicovaginal lavage samples from 208 women, Oligonucleotide probes for 39 different HPV types were used, Both primer sets amplified a wide spectrum of HPV genotypes and detected similar overall prevalences of 45% (94 of 208) and 43% (89 of 208), respectively, The MY-PCR system detected 27 of 30 (90%) samples with multiple HPVtypes, whereas the GP+-PCR system detected 14 of 30 (47%) samples with multiple HPV types, Differences in the detection of HPV types 35, 53, and 61 were noted between the two primer systems, Serial dilution ofplasmid templates indicated a 3-log decrease in the amplification of HPV type 35 by MY-PCR and HPV types 53 and 61 by GP+-PCR. These results indicate that although the MY-PCR and GP+-PCR identified nearly equivalent prevalences of HPV in a set of clinical samples, differences inthe detection of specific types and infections with multiple types were found, Differences in the sensitivities and characteristics of the PCR systems for the detection of HPV within clinical samples should beconsidered when comparing data between studies and/or in designing new studies or clinical trials.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 07:47:49