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Titolo:
Isolation and characterization of mutants from levan-producing Zymomonas mobilis
Autore:
Ananthalakshmy, VK; Gunasekaran, P;
Indirizzi:
Madurai Kamaraj Univ, Sch Biol Sci, Dept Microbial Technol, Madurai 625021, Madurai Kamaraj Univ Madurai Tamil Nadu India 625021 nol, Madurai 625021,
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 2, volume: 87, anno: 1999,
pagine: 214 - 217
SICI:
1389-1723(199902)87:2<214:IACOMF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEVANSUCRASE; STRAINS; FERMENTATION; SUCROSE;
Keywords:
levan; levansucrase; Zymomonas mobilis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Gunasekaran, P Madurai Kamaraj Univ, Sch Biol Sci, Dept Microbial Technol,Madurai 625021, Madurai Kamaraj Univ Madurai Tamil Nadu India 625021 5021,
Citazione:
V.K. Ananthalakshmy e P. Gunasekaran, "Isolation and characterization of mutants from levan-producing Zymomonas mobilis", J BIOSCI BI, 87(2), 1999, pp. 214-217

Abstract

Levan is a fructose polymer with potential importance as a fructose sourceand as a thickening agent in food technology. By N-methyl N'-nitro N-nitroso guanidine (NTG) mutagenesis and selection, two mutants, ZML1 and ZML2, of Zymomonas mobilis B4286 overproducing levan (21.6 and 20.0 g/l at 24 h) were isolated. Despite showing similar rates of sucrose hydrolysis (2.38 g/l/h) in fermentation, these mutants exhibited higher rates of levan production (1.72 and 1.68 g/l/h, respectively) than their parent strain (0.69 g/l/h). In contrast, these mutants showed lower rates of ethanol production (1.06 and 1.55 g/l/h respectively) than the parent strain (3.75 g/l/h). The maximum levansucrase activity in the mutants was higher (30.6 and 36 U/mg, respectively, at 24 h) than in their parent strain (12.6 U/mg). Therefore, theincreased rates of levan production by the mutants are attributed to theirhigher levansucrase activity.

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Documento generato il 02/04/20 alle ore 02:39:46