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Titolo:
Continuous production of L-carnitine with NADH regeneration by a nanofiltration membrane reactor with coimmobilized L-carnitine dehydrogenase and glucose dehydrogenase
Autore:
Lin, SS; Miyawaki, O; Nakamura, K;
Indirizzi:
Univ Tokyo, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 iol Chem, Bunkyo Ku, Tokyo 1138657, Japan
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 3, volume: 87, anno: 1999,
pagine: 361 - 364
SICI:
1389-1723(199903)87:3<361:CPOLWN>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Keywords:
nanofiltration membrane bioreactor; carnitine dehydrogenase; glucose dehydrogenase; NADH regeneration; L-carnitine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Miyawaki, O Univanokyo, Dept Appl Biol Chem, Bunkyo Ku, 1-1-1 Yayoi, Tokyo1138657, Jap Univ Tokyo 1-1-1 Yayoi Tokyo Japan 1138657 Tokyo 1138657, Jap
Citazione:
S.S. Lin et al., "Continuous production of L-carnitine with NADH regeneration by a nanofiltration membrane reactor with coimmobilized L-carnitine dehydrogenase and glucose dehydrogenase", J BIOSCI BI, 87(3), 1999, pp. 361-364

Abstract

L-Carnitine dehydrogenase (CDH) was partially purified from Pseudomonas putida IAM12014 for the stereospecific reduction of 3-dehydrocarnitine to L-carnitine. CDH and glucose dehydrogenase (GDH) were coimmobilized in a nanofiltration membrane bioreactor (NFMBR) for the continuous production of L-carnitine from 3-dehydrocarnitine with NADH regeneration. In the NFMBR, NAD was partially immobilized through rejection by the nanofiltration membrane and effectively regenerated by the conjugation reaction of CDH and GDH. Since 3-dehydrocarnitine was unstable at neutral pH, it was maintained under acidic conditions (pH 0.7) and supplied to the NFMBR separately from the other substrates, glucose and coenzyme NAD. As 50 mM 3-dehydrocarnitine in HCl solution, 0.05 mM NAD, and 100 mM glucose in 0.5 M Tris buffer (pH 8) were continuously supplied to the NFMBR with immobilized CDH (200 U/ml) and GDH (200 U/ml) at the retention time of 80 min and temperature of 25 degrees C,the maximum conversion, reactor productivity, and NAD regeneration number were 78%, 113 g/l/d, and 780, respectively. The half-life of the NFMBR was longer than 500 h.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 09:32:44