Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Potent immunostimulatory dendritic cells can be cultured in bulk from progenitors in normal infant and adult myasthenic human thymus
Autore:
Hill, ME; Ferguson, DJP; Austyn, JM; Newsom-Davis, J; Willcox, HNA;
Indirizzi:
John Radcliffe Hosp, Inst Mol Med, Neurosci Grp, Oxford OX3 9DS, England John Radcliffe Hosp Oxford England OX3 9DS Grp, Oxford OX3 9DS, England John Radcliffe Hosp, Dept Pathol, Oxford OX3 9DS, England John Radcliffe Hosp Oxford England OX3 9DS thol, Oxford OX3 9DS, England John Radcliffe Hosp, Nuffield Dept Surg, Oxford OX3 9DS, England John Radcliffe Hosp Oxford England OX3 9DS Surg, Oxford OX3 9DS, England
Titolo Testata:
IMMUNOLOGY
fascicolo: 2, volume: 97, anno: 1999,
pagine: 325 - 332
SICI:
0019-2805(199906)97:2<325:PIDCCB>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; T-CELLS; PERIPHERAL-BLOOD; SOLUBLE-ANTIGEN; IN-VITRO; RECEPTOR; GRAVIS; ALPHA; DIFFERENTIATION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Willcox, HNA John Radcliffe Hosp, Inst Mol Med, Neurosci Grp, Oxford OX3 9DS, England John Radcliffe Hosp Oxford England OX3 9DS OX3 9DS, England
Citazione:
M.E. Hill et al., "Potent immunostimulatory dendritic cells can be cultured in bulk from progenitors in normal infant and adult myasthenic human thymus", IMMUNOLOGY, 97(2), 1999, pp. 325-332

Abstract

Low density cells can readily be enriched from thymus tissue both of children undergoing cardiac surgery and of older patients with myasthenia gravis, and can be cryostored in bulk. When fresh or thawed cells are cultured with granulocyte-macrophage colony-stimulating factor and stem cell factor with or without tumour necrosis factor-alpha (TNF-alpha), they generate numerous cells with the characteristic ultrastructural, phenotypic and functional properties of dendritic cells. These proved to be very potent, both as stimulators of primary mixed leucocyte responses and as costimulators in oxidative mitogenesis. Especially after exposure to TNF-alpha, these dendritic cells also processed a natural epitope from a 437-residue polypeptide and presented it efficiently to an autoimmune T-cell clone (of T helper type 0 phenotype). Thus, immunostimulatory dendritic cells can be cultured in relative abundance from progenitors in infant and adult human thymus. Both are convenient sources of potent antigen-presenting cells of identifiable origins, e.g. for use in selecting human T-cell lines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 10:07:28