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Titolo: Elafin (elastase-specific inhibitor) has anti-microbial activity against Gram-positive and Gram-negative respiratory pathogens
Autore: Simpson, AJ; Maxwell, AI; Govan, JRW; Haslett, C; Sallenave, JM;
- Indirizzi:
- Univthian,urgh, Sch Med, Rayne Lab, Resp Med Unit, Edinburgh EH8 9AG, Midlo Univ Edinburgh Edinburgh Midlothian Scotland EH8 9AG burgh EH8 9AG, Midlo UnivH8dinburgh, Sch Med, Dept Med Microbiol, Cyst Fibrosis Lab, Edinburgh E Univ Edinburgh Edinburgh Midlothian Scotland EH8 9AG sis Lab, Edinburgh E
- Titolo Testata:
- FEBS LETTERS
fascicolo: 3,
volume: 452,
anno: 1999,
pagine: 309 - 313
- SICI:
- 0014-5793(19990611)452:3<309:E(IHAA>2.0.ZU;2-O
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- LEUKOCYTE PROTEASE INHIBITOR; PSEUDOMONAS-AERUGINOSA; PROTEINASE-INHIBITOR; CYSTIC-FIBROSIS; HUMAN BETA-DEFENSIN-1; CRYSTAL-STRUCTURE; HUMAN SKIN; SEQUENCE; LUNG; ANTILEUKOPROTEASE;
- Keywords:
- elafin; human neutrophil elastase; anti-microbial activity; Pseudomonas aeruginosa; Staphylococcus aureus;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Life Sciences
- Citazioni:
- 42
- Recensione:
- Indirizzi per estratti:
- Indirizzo: Sallenave, JM Univ Edinburgh, Sch Med, Rayne Lab, Resp Med Unit, Teviot Pl, Edinburgh EH8 Univ Edinburgh Teviot Pl Edinburgh Midlothian Scotland EH8 9AG
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- Citazione:
- A.J. Simpson et al., "Elafin (elastase-specific inhibitor) has anti-microbial activity against Gram-positive and Gram-negative respiratory pathogens", FEBS LETTER, 452(3), 1999, pp. 309-313
Abstract
Elafin (elastase-specific inhibitor) is a low molecular weight inhibitor of neutrophil elastase which is secreted in the lung. Using synthetic peptides corresponding to full-length elafin (H2N-(1)AVT.....(95)Q-OH), the NH2-terminal domain (H2N-(1)AVT.....K-50-OH) and the COOH-terminal domain (H(2)N(51)PGS.....(95)Q-OH), demonstrate that elafin's anti-elastase activity resides exclusively in the COOH-terminus. Several characteristics of elafin suggest potential anti-microbial activity. The anti-microbial activity of elafin, and of its two structural domains, was tested against the respiratory pathogensPseudomonas aeruginosa and Staphylococcus aureus. Elafin killed both bacteria efficiently, with 93% killing of P. aeruginosa by 2.5 mu M elafin and 48% killing of S. aureus by 25 mu M elafin. For both organisms, full-length elafin was required to optimise bacterial killing. These findings represent the first demonstration of coexistent anti-proteolytic and anti-microbial functions for elafin. (C) 1999 Federation of European Biochemical Societies.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 19:22:30