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Titolo:
The choice of reference cell in the analysis of kinetic data using BIAcore
Autore:
Ober, RJ; Ward, ES;
Indirizzi:
Univ Texas, SW Med Ctr, Ctr Canc Immunobiol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Ctr Canc Immunobiol, Dallas, TX 75235 USA Univ Texas, SW Med Ctr, Ctr Immunol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Med Ctr, Ctr Immunol, Dallas, TX 75235 USA Univ Texas, Ctr Engn Math EC35, Richardson, TX 75083 USA Univ Texas Richardson TX USA 75083 gn Math EC35, Richardson, TX 75083 USA
Titolo Testata:
ANALYTICAL BIOCHEMISTRY
fascicolo: 1, volume: 271, anno: 1999,
pagine: 70 - 80
SICI:
0003-2697(19990615)271:1<70:TCORCI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMON RESONANCE BIOSENSORS; MASS-TRANSPORT; LIGAND-BINDING;
Keywords:
kinetic constants; surface plasmon resonance; BIAcore; reference cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Ward, ES Univ Texas, SW Med Ctr, Ctr Canc Immunobiol, 6000 Harry Hines Blvd, Dallas, Univ Texas 6000 Harry Hines Blvd Dallas TX USA 75235 lvd, Dallas,
Citazione:
R.J. Ober e E.S. Ward, "The choice of reference cell in the analysis of kinetic data using BIAcore", ANALYT BIOC, 271(1), 1999, pp. 70-80

Abstract

An important step in the analysis of sensorgram data for BIAcore experiments is the subtraction of reference cell data to remove the effects of the hulk shift on the sensorgram of interest. It is shown that this step can introduce errors in the measured kinetic constants. This phenomenon is investigated both theoretically and with experimental data. (C) 1999 Academic Press.

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Documento generato il 25/11/20 alle ore 10:09:15