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Titolo:
Dynamic light-scattering and preliminary crystallographic studies of the sensor domain of the haem-based oxygen sensor FixL from Rhizobium meliloti
Autore:
Miyatake, H; Kanai, M; Adachi, S; Nakamura, H; Tamura, K; Tanida, H; Tsuchiya, T; Iizuka, T; Shiro, Y;
Indirizzi:
RIKEN, Harima Inst, Inst Phys & Chem Res, Sayo, Hyogo 6795143, Japan RIKEN Sayo Hyogo Japan 6795143 hys & Chem Res, Sayo, Hyogo 6795143, Japan Kanagawa Univ, Fac Sci, Kanagawa 2591293, Japan Kanagawa Univ Kanagawa Japan 2591293 v, Fac Sci, Kanagawa 2591293, Japan Tokyo10,d & Dent Univ, Dept Mol Genet, Inst Med Res, Bunkyo Ku, Tokyo 11385 Tokyo Med & Dent Univ Tokyo Japan 1138510 ed Res, Bunkyo Ku, Tokyo 11385
Titolo Testata:
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
, volume: 55, anno: 1999,
parte:, 6
pagine: 1215 - 1218
SICI:
0907-4449(199906)55:<1215:DLAPCS>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIGAND-BINDING; NITRIC-OXIDE; HEME IRON; PROTEIN; PAS; PHOSPHORYLATION; AUTOXIDATION; DIMERIZATION; SUBUNITS; BACTERIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Shiro, Y RIKEN,795143, Inst, Inst Phys & Chem Res, 323-3 Mikazuki Cho, Sayo, Hyogo 6 RIKEN 323-3 Mikazuki Cho Sayo Hyogo Japan 6795143 , Sayo, Hyogo 6
Citazione:
H. Miyatake et al., "Dynamic light-scattering and preliminary crystallographic studies of the sensor domain of the haem-based oxygen sensor FixL from Rhizobium meliloti", ACT CRYST D, 55, 1999, pp. 1215-1218

Abstract

FixL is a transmitter protein in a two-component system which acts as an oxygen sensor when the symbiotic Rhizobia resides in root nodules of host plants. The oxygen;sensor domain of Rhizobium meliloti FixL (RmFixLH) was purified by His-tag affinity and isoelectronic focusing chromatographies, without the use of gelfiltration chromatography. Dynamic light-scattering measurements of RmFixLH thus obtained revealed it to be monodispersive and present as a homodimer in solution. A single crystal of RmFixLH in the met (Fe3+) form was grown in 100 mM acetic acid/NaOH buffer at pH 4.6 in the presence of 200 mM ammonium acetate, using 40%(w/v) PEG 4000 as a precipitant. A crystal of the ferrous CO form of RmFixLH was also prepared by reduction of the met crystal with Na2S2O4 in an atmosphere of CO. The crystals (0.2 x 0.05 x 0.01 mm) belong to the monoclinic system (C2) with unit-cell parameters a = 60.94, b = 37.44, c = 54.14 Angstrom, beta = 115.29 degrees and diffract X-rays to 1.7 Angstrom resolution at station BL44B2 of SPring-8, Japan. Bijvoet difference Patterson maps show a clear peak corresponding to the haem iron in RmFixLH.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 09:43:16