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Titolo:
Optical recording of light-evoked calcium signals in the functionally intact retina
Autore:
Denk, W; Detwiler, PB;
Indirizzi:
AT&T74ell Labs, Lucent Technol, Biol Computat Res Dept, Murray Hill, NJ 079 AT&T Bell Labs Murray Hill NJ USA 07974 tat Res Dept, Murray Hill, NJ 079
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 12, volume: 96, anno: 1999,
pagine: 7035 - 7040
SICI:
0027-8424(19990608)96:12<7035:OROLCS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEINS; SYNAPTIC TRANSMISSION; CELLS; INDICATORS; RODS; CALMODULIN; NEURONS; SPINES; FURA-2; CA2+;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Denk, W AT&T63,ll Labs, Lucent Technol, Biol Computat Res Dept, 600 Mt Ave,Room 1C4 AT&T Bell Labs 600 Mt Ave,Room 1C463 Murray Hill NJ USA 07974 1C4
Citazione:
W. Denk e P.B. Detwiler, "Optical recording of light-evoked calcium signals in the functionally intact retina", P NAS US, 96(12), 1999, pp. 7035-7040

Abstract

Using two-photon excitation of Fluorescent indicator dyes, we measured calcium concentration transients in retinal ganglion and amacrine cells without destroying the light sensitivity of the retina by maximally activating orbleaching the photoreceptors. This allowed an immediate assessment of the cellular morphology and study of the calcium signals evoked by visual stimuli. Calcium dynamics in individual dendritic processes could be examined for extensive periods without deterioration and with little apparent phototoxicity at excitation wavelengths of from 930 to 990 nm. Light-evoked increases in calcium were resolved in ganglion- and amacrine-cell neurites, makingit possible to use optical recording to study the relationship between calcium signaling and retinal function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:24:59