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Titolo:
Co-expression in Xenopus neurons and neuroendocrine cells of messenger RNAhomologues of exocytosis proteins DOC2 and munc18-1
Autore:
Berghs, CAFM; Korteweg, N; Bouteiller, A; Tuinhof, R; Asbreuk, C; Verhage, M; Roubos, EW;
Indirizzi:
CatholicEDniv Nijmegen, Inst Neurosci, Dept Cellular Anim Physiol, NL-6525Catholic Univ Nijmegen Nijmegen Netherlands NL-6525 ED Physiol, NL-6525 Univtrecht,t, Dept Med Pharmacol, Rudolf Magnus Inst Neurosci, NL-3584 CG U Univ Utrecht Utrecht Netherlands NL-3584 CG Inst Neurosci, NL-3584 CG U Univ Rouen, European Inst Peptide Res, F-76821 Mt St Aignan, France Univ Rouen Mt St Aignan France F-76821 Res, F-76821 Mt St Aignan, France
Titolo Testata:
NEUROSCIENCE
fascicolo: 2, volume: 92, anno: 1999,
pagine: 763 - 772
SICI:
0306-4522(1999)92:2<763:CIXNAN>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACKGROUND ADAPTATION; TYROSINE-HYDROXYLASE; PARS-INTERMEDIA; NEUROPEPTIDE-Y; RAT-BRAIN; LAEVIS; SYNTAXIN; IDENTIFICATION; PITUITARY; INTERACTS;
Keywords:
exocytosis; munc18-1; DOC2; co-existence; peptide secretion; Xenopus laevis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Roubos, EW Catholicveldv Nijmegen, Inst Neurosci, Dept Cellular Anim Physiol, Toernooi Catholic Univ Nijmegen Toernooiveld 1 Nijmegen Netherlands NL-6525 ED
Citazione:
C.A.F.M. Berghs et al., "Co-expression in Xenopus neurons and neuroendocrine cells of messenger RNAhomologues of exocytosis proteins DOC2 and munc18-1", NEUROSCIENC, 92(2), 1999, pp. 763-772

Abstract

The proteins munc 18-1 and DOC2 are assumed to play a role in docking of synaptic vesicles in neurotransmitter exocytosis at the presynaptic junction. As the proteins are known to interact, they should co-exist within neurons. We have tested this hypothesis for exocytosis of both classical and peptidergic messengers, by investigating the distribution of the messenger RNAsof munc 18-1 and DOC2 homologues in the brain and pituitary gland of the clawed toad Xenopus laevis, using in situ hybridization. For this purpose wecloned a partial complementary DNA encoding Xenopus unc18 (xunc18) and used a corresponding RNA probe, together with an RNA probe for Xenopus DOC2. At the messenger RNA level DOC2 and xunc18 were found to be expressed throughout the Xenopus brain. All brain nuclei expressing DOC2-messenger RNA showed xunc18-messenger RNA expression as well. Co-expression was shown at the individual cell level in consecutive sections of large-sized neurons. A strong expression was demonstrated in the suprachiasmatic and magnocellular nuclei and in peptidergic endocrine cells in the intermediate and anterior lobes of the pituitary gland, suggesting roles of DOC2 and xunc18 in messenger release from peptidergic secretory systems. Combined in situ hybridization and immunocytochemical analyses show that neuropeptide Y-containing cellsin the suprachiasmatic nucleus also express DOC2 and xunc18 messenger RNAs, Since these cells have a high secretory activity, controlling the activity of the pituitary pars intermedia, the levels of expression of DOC2 and xunc18 may be indicators for neuronal secretory activity. The present data represent the first evidence for the co-existence of DOC2and munc 18-1 and suggest co-ordinate action of these proteins at the level of brain nuclei, individual neurons and endocrine cells. (C) 1999 IBRO. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:24:00