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Titolo:
Phylogenetic composition, spatial structure, and dynamics of lotic bacterial biofilms investigated by fluorescent in situ hybridization and confocal laser scanning microscopy
Autore:
Manz, W; Wendt-Potthoff, K; Neu, TR; Szewzyk, U; Lawrence, JR;
Indirizzi:
Tech Univ Berlin, Dept Microbial Ecol, D-10587 Berlin, Germany Tech Univ Berlin Berlin Germany D-10587 al Ecol, D-10587 Berlin, Germany UFZ,14tr Environm Res Leipzig Halle, Dept Inland Water Res Magdeburg, D-391 UFZ Magdeburg Germany D-39114 le, Dept Inland Water Res Magdeburg, D-391 Natl Water Res Inst, Saskatoon, SK S7N 3H5, Canada Natl Water Res Inst Saskatoon SK Canada S7N 3H5 atoon, SK S7N 3H5, Canada
Titolo Testata:
MICROBIAL ECOLOGY
fascicolo: 4, volume: 37, anno: 1999,
pagine: 225 - 237
SICI:
0095-3628(199905)37:4<225:PCSSAD>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
SULFATE-REDUCING BACTERIA; TARGETED OLIGONUCLEOTIDE PROBES; WHOLE-CELL HYBRIDIZATION; IN-SITU; ACTIVATED-SLUDGE; MICROBIAL BIOFILMS; DRINKING-WATER; IMAGE-ANALYSIS; LABELED OLIGONUCLEOTIDES; MARINE PICOPLANKTON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Manz, W Tech Univ Berlin, Dept Microbial Ecol, D-10587 Berlin, Germany Tech Univ Berlin Berlin Germany D-10587 D-10587 Berlin, Germany
Citazione:
W. Manz et al., "Phylogenetic composition, spatial structure, and dynamics of lotic bacterial biofilms investigated by fluorescent in situ hybridization and confocal laser scanning microscopy", MICROB ECOL, 37(4), 1999, pp. 225-237

Abstract

The phylogenetic composition, three-dimensional structure and dynamics of bacterial communities in river biofilms generated in a rotating annular reactor system were studied by fluorescent in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM). Biofilms grew on independently removable polycarbonate slides exposed in the reactor system with natural river water as inoculum and sole nutrient and carbon source. The microbial biofilm community developed from attached single cells and distinct microcolonies via a more confluent structure characterized by various filamentous bacteria to a mature biofilm rich in polymeric material with fewer cells on a per-area basis after 56 days. During the different stages of biofilm development, characteristic microcolonies and cell morphotypes could be identifiedas typical features of the investigated lotic biofilms. In situ analysis using a comprehensive suite of rRNA-targeted probes visualized individual cells within the alpha-, beta-, and gamma-Proteobacteria as well as the Cytophaga-Flavobacterium group as major parts of the attached community. The relative abundance of these major groups was determined by using digital imageanalysis to measure specific cell numbers as well as specific cell area after in situ probing. Within the lotic biofilm community, 87% of the whole bacterial cell area and 79% of the total cell counts hybridized with a Bacteria specific probe. During initial biofilm development, beta-Proteobacteriadominated the bacterial population. This was followed by a rapid increase of alpha-Proteobacteria and bacteria affiliated to the Cytophaga-Flavobacterium group. In mature biofilms, alpha-Proteobacteria and Cytophaga-Flavobacteria continued to be the prevalent bacterial groups. Beta-Proteobacteria constituted the morphologically most diverse group within the biofilm communities, and more narrow phylogenetic staining revealed the importance of distinct phylotypes within the beta1-Proteobacteria for the composition of themicrobial community. The presence of sulfate-reducing bacteria affiliated to the Desulfovibrionaceae and Desulfobacteriaceae confirmed the range of metabolic potential within the lotic biofilms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 05:58:12