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Titolo:
Functional and molecular expression of intestinal oligopeptide transporter(Pept-1) after a brief fast
Autore:
Thamotharan, M; Bawani, SZ; Zhou, XD; Adibi, SA;
Indirizzi:
Univ0Pittsburgh, Sch Med, Dept Med, Clin Nutr Res Unit, Pittsburgh, PA 1526 Univ Pittsburgh Pittsburgh PA USA 15260 utr Res Unit, Pittsburgh, PA 1526
Titolo Testata:
METABOLISM-CLINICAL AND EXPERIMENTAL
fascicolo: 6, volume: 48, anno: 1999,
pagine: 681 - 684
SICI:
0026-0495(199906)48:6<681:FAMEOI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
BORDER MEMBRANE-VESICLES; RAT SMALL-INTESTINE; GLYCYL-L-PROLINE; PEPTIDE TRANSPORTER; GENE-EXPRESSION; CELL LINE; ABSORPTION; MECHANISM; SUBSTRATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Adibi, SA UPMC Hlth Syst, 200 Lothrop St,MUH E-321, Pittsburgh, PA 15213 USA UPMC Hlth Syst 200 Lothrop St,MUH E-321 Pittsburgh PA USA 15213
Citazione:
M. Thamotharan et al., "Functional and molecular expression of intestinal oligopeptide transporter(Pept-1) after a brief fast", METABOLISM, 48(6), 1999, pp. 681-684

Abstract

The intestinal oligopeptide transporter, cloned as Pept-1, has major rolesin the assimilation of dietary proteins and absorption of peptidomimetic medications. The initial aim of the present experiment was to investigate whether the functional expression of this transporter is affected by dietary intake. Functional expression was determined as the rate of uptake of glycylglutamine (Gly-Gln) by brush-border membrane vesicles (BBMVs) prepared from the jejunum of fed and fasted rats. Surprisingly, the rate of dipeptide uptake was greatly increased after 1 day of fasting. The subsequent aim of the experiment became the investigation of the mechanism of this alteration in transport, which showed that 1 day of fasting increased (1) the maximal Gly-Gln uptake (V-max) by twofold without changing the K-m of Gly-Gln uptake by BBMVs. (2) the amount of intestinal oligopeptide transporter (Pept-1) protein by threefold in the brush-border membrane, and (3) the abundance ofPept-1 mRNA by threefold in the intestinal mucosa. We conclude that 1 day of fasting increases dipeptide transport in rat intestine by increasing thepopulation of Pept-1 in the brush-border membrane. The mechanism appears to be an increase in Pept-1 gene expression. Copyright (C) 1999 by W.B. Saunders Company.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 10:38:42