Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Elimination of duck hepatitis B virus RNA-containing capsids in duck interferon-alpha-treated hepatocytes
Autore:
Schultz, U; Summers, J; Staeheli, P; Chisari, FV;
Indirizzi:
Scripps Clin & Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 Med, La Jolla, CA 92037 USA Univ New Mexico, Dept Mol Genet & Microbiol, Albuquerque, NM 87131 USA Univ New Mexico Albuquerque NM USA 87131 obiol, Albuquerque, NM 87131 USA Univanyeiburg, Inst Med Microbiol & Hyg, Dept Virol, D-79104 Freiburg, Germ Univ Freiburg Freiburg Germany D-79104 ept Virol, D-79104 Freiburg, Germ
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 7, volume: 73, anno: 1999,
pagine: 5459 - 5465
SICI:
0022-538X(199907)73:7<5459:EODHBV>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
AVIAN HEPADNAVIRUS; TRANSGENIC MICE; DNA-SYNTHESIS; ANTIVIRAL ACTIVITIES; SIGNAL-TRANSDUCTION; GENE-TRANSCRIPTION; ENVELOPE PROTEINS; IN-VITRO; REPLICATION; INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Chisari, FV Scrippsolla, & Res Inst, Dept Mol & Expt Med, 10550 N Torrey Pines Rd, La J Scripps Clin & Res Inst 10550 N Torrey Pines Rd La Jolla CA USA 92037
Citazione:
U. Schultz et al., "Elimination of duck hepatitis B virus RNA-containing capsids in duck interferon-alpha-treated hepatocytes", J VIROLOGY, 73(7), 1999, pp. 5459-5465

Abstract

Evidence is presented that the previously cloned type I duck interferon (DuIFN) cDNA encodes a homologue of mammalian interferon-alpha (IFN-alpha). Recombinant DuIFN-alpha was used to study the inhibition of duck hepatitis Bvirus (DHBV) replication in primary hepatocytes in order to determine the IFN-sensitive steps of the virus replication cycle. IFN-treated cells accumulated two- to threefold-lower amounts of viral RNA transcripts early during infection, when IFN was added before virus, This reduction was not due toinhibition of virus entry since initial covalently closed circular DNA levels were not decreased in IFN-treated cells. Interestingly, the inhibitory effect of IFN on viral RNA levels was not observed in cells infected with amutant DHBV that fails to synthesize core protein, suggesting that an uncharacterized core protein-mediated enhancing effect is blocked by IFN, When IFN was added at 4 days postinfection, encapsidated viral RNA pregenomes disappeared from infected cells within 3 days. This depletion was not simply due to conversion of pregenomes to DNA since depletion was not blocked by phosphonoformic acid, an inhibitor of the viral reverse transcriptase. The intracellular concentration of intact nucleocapsids was reduced, suggesting that in the presence of IFN pregenome-containing capsids were selectively depleted in hepatocytes. Thus, two steps in DHBV replication that involve the viral core protein were inhibited by DuIFN-alpha.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 14:53:45