Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
RESPONSE OF MYOCARDIAL CELLULAR-ENERGY METABOLISM TO VARIATION OF BUFFER COMPOSITION DURING OPEN-CHEST EXPERIMENTAL CARDIOPULMONARY-RESUSCITATION IN THE PIG
Autore:
WIKLUND L; RONQUIST G; ROOMANS GM; RUBERTSSON S; WALDENSTROM A;
Indirizzi:
UNIV UPPSALA HOSP,DEPT ANAESTHESIOL S-75185 UPPSALA SWEDEN UPPSALA UNIV,DEPT ANAESTHESIOL UPPSALA SWEDEN UPPSALA UNIV,DEPT CLIN CHEM UPPSALA SWEDEN UPPSALA UNIV,DEPT HUMAN ANAT UPPSALA SWEDEN UPPSALA UNIV,DEPT INTERNAL MED UPPSALA SWEDEN
Titolo Testata:
European journal of clinical investigation
fascicolo: 5, volume: 27, anno: 1997,
pagine: 417 - 426
SICI:
0014-2972(1997)27:5<417:ROMCMT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
X-RAY-MICROANALYSIS; ADENOSINE; ISCHEMIA; ATP; CHARGE; HEART; RELEASE; CELLS; PHOSPHORYLATION; PHOSPHATES;
Keywords:
ALKALINE BUFFERS; CARDIOPULMONARY RESUSCITATION; INTRACELLULAR ELECTROLYTES; MYOCARDIAL METABOLISM; SODIUM BICARBONATE; TRIS BUFFER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
L. Wiklund et al., "RESPONSE OF MYOCARDIAL CELLULAR-ENERGY METABOLISM TO VARIATION OF BUFFER COMPOSITION DURING OPEN-CHEST EXPERIMENTAL CARDIOPULMONARY-RESUSCITATION IN THE PIG", European journal of clinical investigation, 27(5), 1997, pp. 417-426

Abstract

The aim of the present study was to investigate possible relationships in piglets between myocardial energy-related metabolites and intracellular electrolytes during open-chest cardiopulmonary resuscitation (OCCPR) supplemented by the administration of alkaline buffers with varying sodium content. Our hypothesis was that an increasing myocardial intracellular sodium content would decrease the intracellular energy stores. In addition to haemodynamics, acid-base and blood gas variables were analysed, and myocardial biopsies were collected before and during OCCPR as well as after the return of spontaneous circulation. After a period of 4 min of untreated ventricular fibrillation (VF), 25 piglets were randomly allocated to one of four groups: OCCPR with normal saline (n=5); OCCPR with sodium bicarbonate (SE) (n=7); OCCPR with Tris buffer mixture (TBM) (n=7); and a totally untreated control group (n =6). The results showed that 4 min of untreated VF almost eradicated creatine phosphate (CrP) and that the ATP/ADP ratio decreased to 1.5-2.0. During OCCPR with normal saline, the myocardial content of CrP increased, whereas lactate, ATP and ADP levelled off and AMP decreased, causing an increased ATP/ADP ratio. The adenosine and inosine contents increased, whereas inosine monophosphate was unchanged at a low level, the adenosine and inosine contents being inversely correlated with thetotal content of adenine nucleotides. In both buffered groups, the increase in most energy-related metabolites (CrP, ATP, ADP, AMP and the ATP/ADP quotient) was less and in lactate more pronounced than in the group not being buffered, with no difference between the groups receiving SE or TBM. Although the intracellular potassium content was unaltered, the sodium, chloride and calcium concentrations increased, more so in the group receiving SE. The intracellular content of sodium was correlated with that of calcium. Thus, buffering increased the myocardial AMP degradation during OCCPR by increasing the flux via the 5'-nucleotidase reaction, and SE increased the intracellular contents of sodium and calcium to a greater extent than did TBM.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:39:49