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Titolo:
The occurrence of barley mild mosaic virus (BaMMV) in China and the nucleotide sequence of its coat protein gene
Autore:
Zheng, T; Cheng, Y; Chen, JP; Antoniw, JF; Adams, MJ;
Indirizzi:
Zhejiang Acad Agr Sci, Virol Lab, Hangzhou 310021, Peoples R China Zhejiang Acad Agr Sci Hangzhou Peoples R China 310021 1, Peoples R China IARCandthamsted, Crop & Dis Management Dept, Harpenden AL5 2JQ, Herts, Engl IARC Rothamsted Harpenden Herts England AL5 2JQ nden AL5 2JQ, Herts, Engl
Titolo Testata:
JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT
fascicolo: 4, volume: 147, anno: 1999,
pagine: 229 - 234
SICI:
0931-1785(199904)147:4<229:TOOBMM>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATE; BAYMV; RNA-2;
Keywords:
barley mild mosaic virus; China; coat protein gene sequence;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, JP Zhejiang Acad Agr Sci, Virol Lab, Hangzhou 310021, Peoples R China Zhejiang Acad Agr Sci Hangzhou Peoples R China 310021 s R China
Citazione:
T. Zheng et al., "The occurrence of barley mild mosaic virus (BaMMV) in China and the nucleotide sequence of its coat protein gene", J PHYTOPATH, 147(4), 1999, pp. 229-234

Abstract

Barley mild mosaic bymovirus (BaMMV) was detected in 1991, 1992, 1996 and 1997 by enzyme-linked immunosorbent assay and immunosorbent electron microscopy in barley (Hordeum vulgare) from three sites in China (Rudong, Yancheng and Haian). The virus was always present with barley yellow mosaic bymovirus (BaYMV) in the Chinese BaYMV-susceptible cultivar Yanfuaizhao 3, but was not detected in 14 European and two Japanese cultivars that were tested. Electrophoresis of viral RNA and immunoblot analysis indicated that the Chinese isolate of BaMMV contained two RNAs (7.3 and 3.5 kb) and a single coatprotein of approximately 33 kDa. The coat protein gene of the virus was amplified by reverse transcriptase (RT)-polymerase chain reaction (PCR), cloned into the pBluescript (13 +) vector and sequenced. It was identical in length (753 nucleotides; 251 amino acids) to that of isolates from Japan, Korea, Germany, France and the UK. Its homology with the other isolates was 87.8 to 95.2% (nucleotides) and 91.8 to 97.6% (amino acids), and the isolate was most similar to those from Korea and Japan. The results confirmed that the virus detected in China was indeed BaMMV and suggested also that a specific strain of BaMMV has long been established in China.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:07:08