Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Posttreatment with high-dose albumin reduces histopathological damage and improves neurological deficit following fluid percussion brain injury in rats
Autore:
Belayev, L; Alonso, OF; Huh, PW; Zhao, WZ; Busto, R; Ginsberg, MD;
Indirizzi:
Univ Miami, Sch Med, Dept Neurol D4 5, Cerebral Vasc Dis Res Ctr, Miami, FL Univ Miami Miami FL USA 33101 D4 5, Cerebral Vasc Dis Res Ctr, Miami, FL Univ Miami, Sch Med, Dept Neurol, Neurotrauma Res Ctr, Miami, FL 33101 USAUniv Miami Miami FL USA 33101 l, Neurotrauma Res Ctr, Miami, FL 33101 USA
Titolo Testata:
JOURNAL OF NEUROTRAUMA
fascicolo: 6, volume: 16, anno: 1999,
pagine: 445 - 453
SICI:
0897-7151(199906)16:6<445:PWHARH>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBRAL-ARTERY OCCLUSION; COLLOID ONCOTIC PRESSURE; HUMAN EXTRACELLULAR FLUIDS; SERUM-ALBUMIN; MODERATE HYPOTHERMIA; BARRIER DISRUPTION; HYPOCHLOROUS ACID; CRYOGENIC INJURY; DRUG-THERAPY; HEAD-INJURY;
Keywords:
albumin; histopathology; neuroprotection; traumatic brain injury;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Belayev, L Univ0,iami, Sch Med, Dept Neurol D4 5, Cerebral Vasc Dis Res Ctr, POB 01696 Univ Miami POB 016960 Miami FL USA 33101 Dis Res Ctr, POB 01696
Citazione:
L. Belayev et al., "Posttreatment with high-dose albumin reduces histopathological damage and improves neurological deficit following fluid percussion brain injury in rats", J NEUROTRAU, 16(6), 1999, pp. 445-453

Abstract

We have recently shown that high-dose human serum albumin (HSA) therapy confers marked histological protection in experimental middle cerebral arteryocclusion. Thus, the purpose of this study was to determine whether treatment with high-dose HSA would protect in a rat model of traumatic brain injury (TBI). Twenty-four hours prior to TBI, the fluid percussion interface was positioned parasagittally over the right cerebral cortex. On the following day, fasted rats were anesthetized with 3% halothane, 70% nitrous oxide, and 30% oxygen and received right parieto-occipital parasagittal fluid-percussion injury (1.5-2.0 atm). Cranial and rectal temperatures were monitoredthroughout the experiment and held at normothermic levels (36.5-37.5 degrees C) by a warming lamp above the animal's head. The agent (25% human serumalbumin, HSA) or vehicle (sodium chloride 0.9%) was administered i.v. (1% of body weight) 15 min after trauma. Behavioral function was evaluated in all rats before and after TBI (at 2 h, 24 h, 48 h, 72 h, and 7 days). Neurological function was graded on a scale of 0-12 (normal score 0; maximal score = 12). Seven days after TBI, brains were perfusion-fixed, coronal sections at various levels were digitized, and contusion areas in the superficial,middle and deep layers of cortex and in the underlying fimbria were measured. HSA significantly improved the neurological score compared to saline at24 h, 72 h, and 7 days after TBI (6.0 +/- 0.6 [albumin] versus 8.4 +/- 0.5[saline]; 3.6 +/- 0.7 versus 6.8 +/- 1.0; and 2.6 +/- 0.6 versus 5.7 +/- 0.8, respectively; p < 0.05). HSA therapy also significantly reduced total contusion area (0.89 +/- 0.2 versus 1.82 +/- 0.3 mm(2); p = 0.02). Our findings document that high-concentration albumin therapy instituted 15 min after trauma significantly improves the neurological score and reduces histological damage. We believe that this pharmacological agent may have promising potential for the clinical treatment of brain injury.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:32:52