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Titolo:
IN-VIVO COMMITMENT TO SPLICING IN YEAST INVOLVES THE NUCLEOTIDE UPSTREAM FROM THE BRANCH SITE CONSERVED SEQUENCE AND THE MUD2 PROTEIN
Autore:
RAIN JC; LEGRAIN P;
Indirizzi:
INST PASTEUR,DEPT BIOTECHNOL,ARN,LAB METAB,CNRS URA 1149,25 RUE DR ROUX F-75724 PARIS 15 FRANCE INST PASTEUR,DEPT BIOTECHNOL,ARN,LAB METAB,CNRS URA 1149 F-75724 PARIS 15 FRANCE
Titolo Testata:
EMBO journal
fascicolo: 7, volume: 16, anno: 1997,
pagine: 1759 - 1771
SICI:
0261-4189(1997)16:7<1759:ICTSIY>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-MESSENGER-RNA; SMALL NUCLEAR RIBONUCLEOPROTEIN; SACCHAROMYCES-CEREVISIAE; U2 SNRNP; SHUTTLE VECTORS; SR PROTEINS; TACTAAC BOX; U1 SNRNP; IN-VIVO; SPLICEOSOME;
Keywords:
BETA-GALACTOSIDASE; NUCLEAR EXPORT; PRE-MESSENGER-RNA; SACCHAROMYCES-CEREVISIAE; SPLICEOSOME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
J.C. Rain e P. Legrain, "IN-VIVO COMMITMENT TO SPLICING IN YEAST INVOLVES THE NUCLEOTIDE UPSTREAM FROM THE BRANCH SITE CONSERVED SEQUENCE AND THE MUD2 PROTEIN", EMBO journal, 16(7), 1997, pp. 1759-1771

Abstract

Pre-mRNA splicing is a stepwise nuclear process involving intron recognition and the assembly of the spliceosome followed by intron excision. We previously developed a pre-mRNA export assay that allows the discrimination between early steps of spliceosome formation and splicing per se. Here we present evidence that these two assays detect different biochemical defects for point mutations. Mutations at the 5' splice site lead to pre-mRNA export, whereas 3' splice site mutations do not. Ii genetic screen applied to mutants in the branch site region shows that all positions in the conserved TACTAAC sequence are important forintron recognition. An exhaustive analysis of pre-mRNA export and splicing defects of these mutants shows that the in vivo recognition of the branch site region does not involve the base pairing of U2 snRNA with the pre-mRNA. In addition, the nucleotide preceding the conserved TACTAAC sequence contributes to the recognition process. We show that aT residue at this position allows for optimal intron recognition and that in natural introns, this nucleotide is also used preferentially. Moreover, the Mud2 protein is involved in the recognition of this nucleotide, thus establishing a role for this factor in the in vivo splicing pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:17:21