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Titolo:
Signaling complex formation of CD44 with src-related kinases
Autore:
Rozsnyay, Z;
Indirizzi:
GermanGermanyes Ctr, Dept Tumor Progress & Immune Def, D-69120 Heidelberg,German Canc Res Ctr Heidelberg Germany D-69120 Def, D-69120 Heidelberg,
Titolo Testata:
IMMUNOLOGY LETTERS
fascicolo: 1, volume: 68, anno: 1999,
pagine: 101 - 108
SICI:
0165-2478(19990503)68:1<101:SCFOCW>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-TYROSINE KINASE; PHOSPHORYLATED IG-ALPHA; PLASMA-MEMBRANE DOMAINS; T-CELL ACTIVATION; CYTOPLASMIC DOMAIN; RECEPTOR COMPLEX; CD5 ACTS; ASSOCIATION; SUBSTRATE; P56(LCK);
Keywords:
CD44; src-like kinases; constitutive association;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Rozsnyay, Z Inst Rech Jouveinal, Pk Davis,BP 100, F-94265 Fresnes, France Inst Rech Jouveinal Pk Davis,BP 100 Fresnes France F-94265 ce
Citazione:
Z. Rozsnyay, "Signaling complex formation of CD44 with src-related kinases", IMMUNOL LET, 68(1), 1999, pp. 101-108

Abstract

The complex formation of murine CD44 with the src-like protein tyrosine kinases, lck and lyn, was investigated. In accordance with previous observations, stable CD44-lck and CD44-lyn complexes were detected in nonstimulated lymphoid T- and B-cells, respectively. In addition, a direct modulation of lck and lyn by CD44 was observed as revealed by the CD44-dependent translocation of these enzymes to the Triton X-100 resistant cell fraction. To clarify which receptor domain is responsible for the association, peptide binding assays were performed. Interestingly, the synthetic peptide pCD44 (ILAVCIAVNSRRR), which corresponds to the plasma membrane-cytoplasmic interface region of murine CD44, exhibited a high capacity to bind lck and lyn. A single amino acid modification in the position of the cysteine residue completely abolished this interaction, while the truncation of the three tandem arginines significantly decreased it. Remarkably, similar sequences were foundin a number of other molecules including subunits of receptors recognizingantigens, immunoglobulins, extracellular matrix components, accessory molecules, cytokines and also in certain viral gene products. Synthetic peptides corresponding to the homologous regions found in CD28 and Fc epsilon RI beta were also studied and comparable lck-lyn-binding potentials were detected. These data suggest a novel interaction between sie-family kinases and CD44, CD28, Fc alpha RI beta, and provide a simple model for the associationof sic-like kinases with transmembrane proteins. (C) 1999 Elsevier ScienceB.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 02:11:21