Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Purification of a candidate gonadotrophin surge attenuating factor from human follicular fluid
Autore:
Pappa, A; Seferiadis, K; Fotsis, T; Shevchenko, A; Marselos, M; Tsolas, O; Messinis, IE;
Indirizzi:
Univ Thessalia, Sch Med, Dept Obstet & Gynaecol, Larissa 41222, Greece Univ Thessalia Larissa Greece 41222 et & Gynaecol, Larissa 41222, Greece Univ Ioannina, Sch Med, Dept Pharmacol, GR-45110 Ioannina, Greece Univ Ioannina Ioannina Greece GR-45110 rmacol, GR-45110 Ioannina, Greece Univ Ioannina, Sch Med, Biol Chem Lab, GR-45110 Ioannina, Greece Univ Ioannina Ioannina Greece GR-45110 em Lab, GR-45110 Ioannina, Greece EMBL, Prot & Peptide Grp, Heidelberg, Germany EMBL Heidelberg GermanyEMBL, Prot & Peptide Grp, Heidelberg, Germany
Titolo Testata:
HUMAN REPRODUCTION
fascicolo: 6, volume: 14, anno: 1999,
pagine: 1449 - 1456
SICI:
0268-1161(199906)14:6<1449:POACGS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
SELF-PRIMING ACTION; STIMULATING-HORMONE; INHIBITING FACTOR; FACTOR GNSAF; SUPEROVULATED WOMEN; SERUM-ALBUMIN; SECRETION; PROTEINS; INVIVO; LHRH;
Keywords:
gonadotrophin surge attenuating factor; human follicular fluid; luteinizing hormone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Messinis, IE Univ Thessalia, Sch Med, Dept Obstet & Gynaecol, 22 Papakiriazi St, Larissa Univ Thessalia 22 Papakiriazi St Larissa Greece 41222 arissa
Citazione:
A. Pappa et al., "Purification of a candidate gonadotrophin surge attenuating factor from human follicular fluid", HUM REPR, 14(6), 1999, pp. 1449-1456

Abstract

Gonadotrophin surge attenuating factor (GnSAF) is a new non-steroidal ovarian substance, different from inhibin, which attenuates the pre-ovulatory luteinizing hormone (LH) surge in superovulated women. Human follicular fluid (FF) was used as a source for the isolation of GnSAF, the activity of which was monitored in an in-vitro pituitary bioassay. Primary rat pituitary cells were incubated with test substances for 48 h and subsequently washed and incubated with 0.1 mu mol/l gonadotrophin releasing hormone (GnRH) plus test substances for 4 h. GnSAF activity was expressed as the reduction of GnRH-induced LH secretion in the 4 h incubation. GnSAF was purified from 250mi of FF which was heat-treated at 80 degrees C for 5 min. Heparin-sepharose chromatography, Con-A sepharose chromatography, reversed-phase high-performance liquid chromatography (HPLC) and preparative native gel electrophoresis were used for GnSAF fractionation. Using these purification steps, we have obtained an apparently homogeneous preparation that stains as a singleband on sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis, GnSAF has an apparent molecular weight of 12.5 kDa and was identified by amino acid sequence (mass spectrometry) to be the C-terminal fragment of human serum albumin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:32:34