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Titolo:
A novel apoptosis-like pathway, independent of mitochondria and caspases, induced by curcumin in human lymphoblastoid T (Jurkat) cells
Autore:
Piwocka, K; Zablocki, K; Wieckowski, MR; Skierski, J; Feiga, I; Szopa, J; Drela, N; Wojtczak, L; Sikora, E;
Indirizzi:
M Nencki Inst Expt Biol, Dept Cellular Biochem, PL-02093 Warsaw, Poland M Nencki Inst Expt Biol Warsaw Poland PL-02093 , PL-02093 Warsaw, Poland Drug Inst, Flow Cytometry Lab, Warsaw, Poland Drug Inst Warsaw PolandDrug Inst, Flow Cytometry Lab, Warsaw, Poland Univ Wroclaw, Inst Biochem, PL-50138 Wroclaw, Poland Univ Wroclaw Wroclaw Poland PL-50138 t Biochem, PL-50138 Wroclaw, Poland Univ Wroclaw, Dept Immunol, PL-50138 Wroclaw, Poland Univ Wroclaw Wroclaw Poland PL-50138 t Immunol, PL-50138 Wroclaw, Poland
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 2, volume: 249, anno: 1999,
pagine: 299 - 307
SICI:
0014-4827(19990615)249:2<299:ANAPIO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERMEABILITY TRANSITION PORE; CYTOCHROME-C RELEASE; DIETARY CURCUMIN; INTRACELLULAR GLUTATHIONE; COLON CARCINOGENESIS; TUMOR PROMOTION; RAT THYMOCYTES; LEUKEMIA-CELLS; MOUSE SKIN; BCL-2;
Keywords:
apoptosis; curcumin; ultraviolet light C; mitochondrial membrane potential; caspase-3; Jurkat cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Sikora, E M,Nencki Inst Expt Biol, Dept Cellular Biochem, Pasteura 3, PL-02093 Warsaw M Nencki Inst Expt Biol Pasteura 3 Warsaw Poland PL-02093 arsaw
Citazione:
K. Piwocka et al., "A novel apoptosis-like pathway, independent of mitochondria and caspases, induced by curcumin in human lymphoblastoid T (Jurkat) cells", EXP CELL RE, 249(2), 1999, pp. 299-307

Abstract

We have shown previously [E, Sikora, A Bielak-Zmijewska, K. Piwocka, J. Skierski, and E. Radziszewska (1997) Biochem. Pharmacol. 54, 899-907] that curcumin prevents formation of oligonucleosomal DNA fragmentation in rat thymocytes and human leukemic T lymphocytes Jurkat cells induced to undergo apoptosis. In this paper we show that 50 mu M curcumin by itself induces cell death in Jurkat cells, but its symptoms differ from those observed after a short ultraviolet (uv) irradiation. Ultraviolet-irradiated Jurkat cells displayed typical symptoms of apoptosis: morphological changes, internucleosomal and high-molecular-weight DNA fragmentation, formation of sub-G, fractions in DNA content frequency histograms, and dissipation of the mitochondrial transmembrane electric potential (Delta psi). In contrast, curcumin-treated Jurkat cells exhibited DNA splitting into high-, but not low-, molecular-weight fragments. These cells retained their high mitochondrial Delta psi and the content of Ca2+ in endoplasmic reticulum stores remained at the level typical for untreated cells. The frequency of opening of the mitochondrial permeability transition pores in curcumin-treated cells was decreased compared to the controls, whereas uv irradiation made these pores completely open. Curcumin did not produce any change in the activity of caspase-3, whereas uv irradiation considerably activated this protease. The morphology ofcurcumin-treated cells displayed chromatin condensation, which was insensitive to the caspase inhibitor z-VAD-fmk, but no formation of typical apoptotic bodies, as was the case after uv irradiation. In contrast to uv-irradiated cells, curcumin-treated Jurkat cells considerably increased the level of Bcl-2. It is concluded that the programmed cell death induced by curcuminin Jurkat cells differs from "classical" by the lack of mitochondrial depolarization and of the involvement of caspases. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 05:48:06