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Titolo:
Human bronchial smooth muscle cells in culture produce stem cell factor
Autore:
Kassel, O; Schmidlin, F; Duvernelle, C; Gasser, B; Massard, G; Frossard, N;
Indirizzi:
INSERM, U425, Fac Pharm, F-67401 Illkirch Graffenstaden, France INSERM Illkirch Graffenstaden France F-67401 kirch Graffenstaden, France Hop Univ, Inst Pathol Anat, Strasbourg, France Hop Univ Strasbourg France p Univ, Inst Pathol Anat, Strasbourg, France Hop Univ, Serv Chirurg Thorac, Strasbourg, France Hop Univ Strasbourg France niv, Serv Chirurg Thorac, Strasbourg, France
Titolo Testata:
EUROPEAN RESPIRATORY JOURNAL
fascicolo: 5, volume: 13, anno: 1999,
pagine: 951 - 954
SICI:
0903-1936(199905)13:5<951:HBSMCI>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-KIT LIGAND; HUMAN MAST-CELL; EXPRESSION; ACTIVATION; GENE; CYTOKINES;
Keywords:
asthma; mast cells; smooth muscle; stem cell factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Kassel, O INSERM, U425, Fac Pharm, BP 24, F-67401 Illkirch Graffenstaden, France INSERM BP 24 Illkirch Graffenstaden France F-67401 aden, France
Citazione:
O. Kassel et al., "Human bronchial smooth muscle cells in culture produce stem cell factor", EUR RESP J, 13(5), 1999, pp. 951-954

Abstract

A mast cell infiltration of the bronchial smooth muscle layer has been reported in patients sensitized to common allergens. Stem cell factor (SCF) isa chemotactic and survival factor for mast cells. SCF is expressed as a soluble (sSCF) and a membrane-bound (mSCF) form, after alternative splicing of the exon encoding the proteolytic cleavage site. SCF expression by human bronchial smooth muscle cells in culture was evaluated, comparing it to that of human lung fibroblasts in culture.sSCF released in the culture supernatant was assessed by an enzyme-linked immunosorbent assay. Total SCF messenger ribonucleic acid (mRNA) was measured by competitive polymerase chain reaction (PCR) after reverse transcription. Expression of the two forms of SCF mRNA was assessed by PCR, with primers spanning the alternatively spliced exon. Smooth muscle cells produced sSCF (21.9+/-2.6 pg.mL(-1)), although at lower levels than fibroblasts (35.9+/-3.5 pg.mL(-1)); the expression of total SCF mRNA was also at lower levels than in fibroblasts (8.6+/-0.2 and 19.0+/-2.0 amol.fmol glyceraldehyde 3-phosphate dehydrogenase complementary deoxyribonucleic acid(-1), respectively). However, smooth muscle cells expressed proportionally more (1.7-fold) mSCF mRNA than did fibroblasts. In conclusion, this study shows that bronchial smooth muscle cells expressstem cell factor, with a relatively high expression of membrane-bound stemcell factor. This might be related to the presence of mast cells within the bronchial smooth muscle layer, ie. at the site of bronchoconstriction, with possible implications in the pathophysiology of asthma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/21 alle ore 22:37:57