Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
8-hydroxy-2 '-deoxyguanosine, a major mutagenic oxidative DNA lesion, and DNA strand breaks in nasal respiratory epithelium of children exposed to urban pollution
Autore:
Calderon-Garciduenas, L; Lian, WW; Zhang, YJ; Rodriguez-Alcaraz, A; Osnaya, N; Villarreal-Calderon, A; Santella, RM;
Indirizzi:
Univ N Carolina, Curriculum Toxicol, Chapel Hill, NC USA Univ N Carolina Chapel Hill NC USA rriculum Toxicol, Chapel Hill, NC USA Inst Nacl Pediatria, Mexico City, DF, Mexico Inst Nacl Pediatria Mexico City DF Mexico atria, Mexico City, DF, Mexico Columbia Univ, Div Environm Hlth Sci, New York, NY USA Columbia Univ New York NY USA v, Div Environm Hlth Sci, New York, NY USA Soc Mex ORL & CCC, Mexico City, DF, Mexico Soc Mex ORL & CCC Mexico City DF Mexico L & CCC, Mexico City, DF, Mexico
Titolo Testata:
ENVIRONMENTAL HEALTH PERSPECTIVES
fascicolo: 6, volume: 107, anno: 1999,
pagine: 469 - 474
SICI:
0091-6765(199906)107:6<469:8'AMMO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIESEL EXHAUST PARTICLES; MEXICO-CITY; LUNG-CELLS; AIRWAY EPITHELIUM; REACTIVE OXYGEN; FREE-RADICALS; BASE DAMAGE; RAT-LIVER; IN-VITRO; CANCER;
Keywords:
air pollution; biomarker; children; DNA damage; DNA strand breaks; 8-hydroxy-2 '-deoxyguanosine; Mexico City; nasal epithelium; oxidative DNA damage; reactive nitrogen species; reactive oxygen species;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Calderon-Garciduenas, L US EPA, MD 58D, Res Triangle Pk, NC 27711 USA US EPA MD 58D Res Triangle Pk NC USA 27711 711 USA
Citazione:
L. Calderon-Garciduenas et al., "8-hydroxy-2 '-deoxyguanosine, a major mutagenic oxidative DNA lesion, and DNA strand breaks in nasal respiratory epithelium of children exposed to urban pollution", ENVIR H PER, 107(6), 1999, pp. 469-474

Abstract

Southwest metropolitan Mexico City children are repeatedly exposed to highlevels of a complex mixture of air pollutants, including ozone, particulate matter, aldehydes, metals, and nitrogen oxides. We explored nasal cell 8-hydroxy-2'-deoxyguanosine (8-OHdG), a major mutagenic lesion producing G-->T transversion mutations, using an immunohistochemical method, and DNA single strand breaks (ssb) using the single cell gel electrophoresis assay as biomarkers of oxidant exposure. Nasal biopsies from the posterior inferior turbinate were examined in children in grades one through five, including 12controls from a low-polluted coastal town and 87 Mexico City children. Each biopsy was divided for the 8-OHdG and DNA ssb assays. There was an age-dependent increase in the percentage of nasal cells with DNA tails > 10 mu m in Mexico City children: 19 +/- 9% for control cells, and 43 +/- 4, 50 +/- 16, 56 +/- 17, 60 +/- 17 and 73 +/- 14%, respectively, for first through fifth graders (p < 0.05). Nasal ssb were significantly higher in fifth graders than in first graders (p < 0.05). Higher levels (2.3- to 3-fold) of specific nuclear staining for 8-OHdG were observed in exposed children as compared to controls (p < 0.05). These results suggest that DNA damage is presentin nasal epithelial cells in Mexico City children. Persistent oxidative DNA damage may ultimately result in a selective growth of preneoplastic nasalinitiated cells in this population and the potential for nasal neoplasms may increase with age. The combination of 8-OHdG and DNA ssb should be useful for monitoring oxidative damage in people exposed to polluted atmospheres.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 21:38:31