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Titolo:
Flow cytometry of cerebrospinal fluid (CSF) lymphocytes: Alterations of blood CSF ratios of lymphocyte subsets in inflammation disorders of human central nervous system (CNS)
Autore:
Kleine, TO; Albrecht, J; Zofel, P;
Indirizzi:
Univ033rburg, Med Zentrum Nervenheilkunde, Funktionsbereich Neurochem, D-35 Univ Marburg Marburg Germany D-35033 e, Funktionsbereich Neurochem, D-35 Univ Marburg, Abt Anwendung, Hochschulrechenzentrum, Marburg, Germany UnivMarburg Marburg Germany , Hochschulrechenzentrum, Marburg, Germany
Titolo Testata:
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
fascicolo: 3, volume: 37, anno: 1999,
pagine: 231 - 241
SICI:
1434-6621(199903)37:3<231:FCOCF(>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; BRAIN-BARRIER BBB; MULTIPLE-SCLEROSIS; PERIPHERAL-BLOOD; UP-REGULATION; ADHESION MOLECULES; CHOROID-PLEXUS; CELLS; EXPRESSION; ENDOTHELIUM;
Keywords:
flow cytometry; cerebrospinal fluid (CSF); human lymphocyte subsets; blood-brain barrier; blood-CSF barrier; inflammations;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Kleine, TO Univlfarburg, Med Zentrum Nervenheilkunde, Funktionsbereich Neurochem, Rudo Univ Marburg Rudolf Bultmann Str 8 Marburg Germany D-35033 udo
Citazione:
T.O. Kleine et al., "Flow cytometry of cerebrospinal fluid (CSF) lymphocytes: Alterations of blood CSF ratios of lymphocyte subsets in inflammation disorders of human central nervous system (CNS)", CLIN CH L M, 37(3), 1999, pp. 231-241

Abstract

Flow cytometry was adapted to measure lymphocytes in human cerebrospinal fluid (CSF). The method was sufficiently precise, reproducible and accurate despite low cell counts. In lumbar CSF of controls with 500 to 3500 (10(3)/I) leukocytes, lymphocyte counts correlated with those in corresponding venous blood: blood/CSF ratios of approximate to 2000 : 1 were found for totalT cells (CD3(+)) and CD3(+) HLA-DR-, CD3(+)4(+), CD3(+)8(+) subsets, ratios were increased for the lymphocyte subsets CD3(+) HLA-DR+ less than or equal to; CD3(+)16(+)56(+) < CD16(+)56(+)3(-) < CD8(+)3(-) much less than CD19(+); CD8(+)4(+) ratio was half of CD3(+) ratio. Data indicate selective barriers (blood-brain and blood-CSF barriers) tio blood lymphocyte subsets which favor the transfer of T subsets. Correlation of the subset ratios to theCD3(+) ratio indicates distinct barrier properties which changed differently with acute and subacute inflammations and neuroimmunological diseases ofcentral nervous system (CNS) in lumbar or ventricular CSF, but not with simple protein barrier disturbance. HLA DR+ T ratios were higher than HLA DR-T ratios only with controls and some neuroimmunological diseases. Lymphocyte barrier characteristics were related tot protein leakage situated at thesame barriers, indicating far the lymphocyte subsets selective transfer routes in control subjects and non-selective routes in patients with CNS inflammation where altered ratios revealed a mixture of both routes.

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Documento generato il 24/01/20 alle ore 12:56:19