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Titolo:
Immunostimulatory CpG motifs trigger a T helper-1 immune response to humanimmunodeficiency virus type-1 (HIV-1) gp160 envelope proteins
Autore:
Deml, L; Schirmbeck, R; Reimann, J; Wolf, H; Wagner, R;
Indirizzi:
Univ Regensburg, Inst Med Microbiol, D-8400 Regensburg, Germany Univ Regensburg Regensburg Germany D-8400 ol, D-8400 Regensburg, Germany Univ Ulm, Inst Med Microbiol & Immunol, Ulm, Germany Univ Ulm Ulm Germany iv Ulm, Inst Med Microbiol & Immunol, Ulm, Germany
Titolo Testata:
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
fascicolo: 3, volume: 37, anno: 1999,
pagine: 199 - 204
SICI:
1434-6621(199903)37:3<199:ICMTAT>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTERIAL-DNA; INTERFERON-GAMMA; B-CELL; ADJUVANTS; ANTIGEN; INTERLEUKIN-12; IMMUNIZATION; PARTICLES; MURINE; OLIGODEOXYNUCLEOTIDES;
Keywords:
HIV gp160; CpG motifs; aluminium hydroxide; adjuvants; cytokines; T helper-1 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Wagner, R Klinikum053gensburg, Inst Med Microbiol, Franz Josef Strauss Allee 11, D-93 Klinikum Regensburg Franz Josef Strauss Allee 11 Regensburg Germany D-93053
Citazione:
L. Deml et al., "Immunostimulatory CpG motifs trigger a T helper-1 immune response to humanimmunodeficiency virus type-1 (HIV-1) gp160 envelope proteins", CLIN CH L M, 37(3), 1999, pp. 199-204

Abstract

Bacterial DNA sequences containing unmethylated CpG motifs have recently been proposed to exhibit immunostimulatory effects on B-, T- and NK cells, leading to the induction of humoral and cell-mediated immune responses. In the present study we investigated the immunomodulatory effects of a CpG-containing oligodeoxynucleotide (CpG ODN) to the HIV-1 gp160 envelope (Env) protein in the BALB/c mouse model. Priming and boosting of mice with gp160 adsorbed to aluminium hydroxide (Alum) induced a typical T helper-2 (Th2)-dominated immune response with high titers of gp160-specific immunoglobulin (Ig)G1 isotypes but a weak IgG2a response. Specifically re-stimulated splenocytes from these mice predominantly secreted interleukin (IL)-5 but only minute amounts of interferon-gamma (IFN-gamma) upon specific re-stimulation. Incontrast, a boost immunisation of gp160/Alum primed mice with a gp160/Alum/CpG combination resulted in a seven times higher production of IgG2a antibodies, without affecting the titers of IgG1 isotypes. Furthermore, approximately 10-fold increased levels of IFN-gamma, but significantly reduced amounts of IL-5, were secreted from gp160-restimulated splenic cells. A furthergreater than 30-fold increase in the levels of specific IgG2a responses and a substantially elevated secretion of IFN-gamma were observed when the mice received gp160/Alum/CpG combinations for priming and boost injections. Thus, CpG ODNs are useful as an adjuvant to induce a typical Th0/Th1 response to HIV gp160 proteins. However, despite the induction of a more Th1-like immune response, gp160/Alum/CpG combinations were not sufficient to prime an Env-specific cytotoxic T-cell (CTL) response.

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Documento generato il 21/09/20 alle ore 18:51:31